Gharabi Dhia¹, Hassani Abdelkrim^1*, Hellal Benchaben², Labdaoui Djamel³

1Laboratory of Agrobiotechnology and Nutrition in dry areas, Ibn Khaldoun University, Tiaret, Algeria

²Laboratory of Geomatics and Sustainable Development, Ibn Khaldoun University, Tiaret, Algeria

³Laboratory of plant protection, Abdelhamid Ibn Badis University, Mostaganem, Algeria

Abstract

The objective of this work is to determine the effect of salinity on the morpho-physiological behavior of young olive plants in cuttings and grafted plants, including two local varieties (Chemlal and Sigoise) and two introduced varieties (Manzanilla and Arbiquina). The experiment took place in a greenhouse with a well-controlled condition. The experimental plan is in a complete randomization of two factors (salinity and varieties); plants without NaCl application were used as control. The plant material (young 2-year-old olive seedlings) was selected and brought from a crop nursery. The experimentation started by the irrigation of the control plant with a nutrient solution. About the salinity treatment, the tree seedlings received a nutrient solution fortified by the addition of 100mM of NaCl. Four repetitions are being done according to the field capacity. The study covered the variation of the relative water content in leaves as well as the leaf surface, stomata density, stomatal size and the wax rate on the upper foliar epidermis. RWC leaf area and stomatal density of the treated plants decreased compared to the control. On the other hand, the wax level increases in the case of salt stress compared to the control, both for the grafted plants and for the plants not grafted.

Keywords: Olive-tree, Local varieties, Salt stress, RWC, Wax rate  

Dalia Sukmawati*, Nur Saidah, Tri Handayani K, Sri Rahayu

Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Campus B, Jl. Pemuda No. 10 Rawamangun, East Java, Indonesia

Abstract

Fungi can cause contamination in animal feed. Contamination of the feed may result in damage to feed quality and decrease the health of livestock. The aim of this study was to isolate and identify fungi strains in cage- and warehouse-based chicken feed. Fungal isolates was collected using the dilution plating method. A sample was taken from the cage and feed in Tegal village, Bogor, West Java. The numbers of sample taken were 10 g of each plot. The isolation, identification and characterization of mold, was based on its phenotype macroscopic and microscopic. Forty-six molds [7 sourced from cages (15.21%) and 39 from warehouses (84.78%)] were isolated from 10 samples of the cage and warehouse-based chicken feed. The selection of representative mold isolates amounted to 22 isolates, consisting of four mold isolates from cages and 18 isolates from the warehouse. Six genera were identified, based on their morphological characteristics: Aspergillus (3 isolates), Penicillium (8 isolates), Fusarium (3 isolates), Trichoderma (3 isolates), Cladosporium (1 isolate), Paecilomyces (2 isolates) and Mycelia sterilia (2 isolates). The highest occurrence of mold isolates in chicken feed was Penicillium (36.36%), and the lowest of it was Cladosporium (4.54%).  The findings are in line with the information about occurrence fungi in the chicken feed of poultry. These results showed that a potential exists for fungi contamination in chicken feed can be found at cage- and warehouse poultry feeds.  Occurrence of fungi gives information to farmers to prevent a health of their livestock from excessively contaminated fungi genera Penicillium. Controlling as prevented by early detection or visual inspection and good management is a better choice compared to curing.

Keywords: Fungal, Cladosporium, Isolation, Penicillium, Poultry feed contamination, Warehouse

Farhana Hussain¹, Md. Reazul Karim^*1, Fahmida Hossain², Faisal Hasan³

¹Department of Microbiology, University of Chittagong, Chittagong-4331, Bangladesh

²Department of Chemistry, Jahangirnagar University, Savar, Dhaka, Bangladesh

³University of Science and Technology, Chittagong, Chittagong-4202, Bangladesh

Abstract

Water pollution due to the desertion of enormous volume of effluent like synthetic dyes mostly azo dyes from the textile dyeing industries has become an alarming trend in the present world.  Biological degradation of synthetic dye is gaining popularity because of its cost effectiveness and ecofriendly procedure. Hence, the present study was aimed to isolate and identify the indigenous azo dye decolorizing bacteria from dyeing industry effluent and to assay their dye decolorization potential in order to use them as an efficient bio-agent for decolorizing and mineralizing toxic azo dyes. Thirty indigenous bacterial colonies were isolated initially from which eight bacterial isolates were selected by further screening method, exhibiting 10% Novacron Red SB decolorization in semi-solidified screening medium. The decolorizer isolates were identified upto species as Bacillus alvei, Bacillus polymyxa, Corynebacterium rathayi, Staphylococcus aureus, Zymomonas anaerobia, Bacillus megaterium, Aerobacter aerogenes and Micrococcus conglomeratus on the basis of their morphological, cultural, physiological and biochemical characteristics. The maximum decolorization by the isolates was achieved with 5% dye concentration at 37°C temperature and pH 9 and peptone as co-substrate. So, this study demonstrates that the selected eight indigenous isolates can be used as efficient biological agent for the removal of toxic industrial novacron dyes by maintaining the above mentioned optimum value of the process parameter.

Keywords: Water pollution, Dyeing industry effluents, Azo dyes, Decolorization, Biodegradation    

Raja Nur Rahifah Inani¹, Afzan Mat Yusof^2,3*

¹Department of Biomedical Science, Kulliyyah of Allied Health Sciences, International Islamic University Malaysia, Jalan Sultan Ahmad Shah, Bandar Indera Mahkota, 25200 Kuantan, Pahang, Malaysia

²Department of Basic Medical Sciences, Kulliyyah of Nursing, International Islamic University Malaysia, Jalan Hospital Campus, 25100 Kuantan, Pahang, Malaysia

³Integrated Cellular and Molecular Biology Cluster (iMolec), International Islamic University Malaysia, Jalan Sultan Ahmad Shah, Bandar Indera Mahkota, 25200 Kuantan, Pahang, Malaysia

Abstract

Gastrointestinal parasitic infection is a major concern to livestock sector as it causes severe diseases and death to the goats. This study was designed to measure the occurrence of gastrointestinal parasitic infections in goats and its association with the seasonal variations in a commercial farm located in Kuantan, Malaysia. A total of 480 faecal samples were collected randomly from 40 goats in 12 months. All samples were processed under faecal flotation technique for morphology and size of the egg/oocyst of parasites and McMaster modified technique for the OPG/EPG of parasites. Coproculture examination was performed to identify the species of strongyles infected goats in the present study. The obtained data were analysed by using SPSS software under independent t-test and chi-square test. Out of 480 faecal samples, 429 samples were positive and give the overall prevalence 89.4%. The infection rate was significantly higher in a dry climate (92.1%) than wet climate (85.5%). The mean egg counts of strongyles, Strongyloides and Enterobius were significantly higher in a dry climate than wet climate (P<0.005). Meanwhile, the mean egg or oocyst counts of Trichuris, Moniezia and Eimeria were higher in wet climate than dry climate, but the results were not significant. The findings from the present study suggested that appropriate preventive measures such as maintaining cleanliness and provide appropriate anthelminthic to goats need to be applied to the goats, farms and farm workers to prevent the widespread of infections to goats and humans.

Keywords: Gastrointestinal parasitic infection, Goats, Seasonal, Livestock, Malaysia

Muhammad Aslam, Khola Rafique*

Pest Warning and Quality Control of Pesticides, Department of Agriculture, Govt. of Punjab, Pakistan

Abstract

To monitor Tikka or Cercospora leaf spot disease of groundnut, surveys were carried out in the groundnut areas of four major districts of Pothwar region viz. Chakwal, Attock, Jhelum and Rawalpindi during main crop season in 2017. To assess prevalence and incidence of Tikka disease, 997 farmer’s fields were visited. Clear disease symptoms were noted in the fields during the season. The disease prevalence ranged from 7.85 to 45% where highest prevalence was recorded in district Jhelum (45%) and lowest in Attock (7.85%). The disease incidence also varied and ranged from 9.35 to 22.48%. The highest mean disease incidence (22.48%) was observed in district Chakwal, whereas the lowest (10.57%) was observed in district Rawalpindi. The results of the study indicated that Tikka disease is significantly distributed in all the major groundnut producing districts of Pothwar region therefore, timely and possible management strategies are of vital importance to control this potential threat.

Keywords: Cercospora, Tikka disease, Leaf spot, Incidence, Prevalence

Johanna Suek¹*, Irham², Slamet Hartono², Lestari Rahayu Waluyati²

¹Graduate Student of Department of Agricultural Economic, Agricultural Faculty, Gajah Mada University, Jl. Flora Kompleks Bulaksumur, Yogyakarta, DIY 55281, Indonesia.

²Department of Agribusiness, Faculty of Agriculture, Nusa Cendana University, Jl. Adisucipto Penfui, Kupang, East Nusa Tenggara Province 85001, Indonesia

Abstract

A traditional agroforestry named mamar has been commonly practiced by small-scale farmers of western part of Timor Island, East Nusa Tenggara Province. Mamar system is considered by many as an environmentally sound agriculture practice. However, as for other agriculture systems, the productivity of mamar system is also prone to risks caused by many internal and external factors. This study was designed to determine factors affecting the production and risk production of mamar. This research was conducted in four mamar-containing regencies of western Timor Island, East Nusa Tenggara Province. Eleven villages which have at least 30% mamar farmer households were selected from the regencies. From each village, 30 farmers were randomly selected and interviewed based on structured questionnaires. Multiple regression models ran with Eviews 6 software were utilized. Results showed that: land area, number of kinds of annual food, perennial cash, forestry, and fodder crops, number of animals reared, organic fertilizer usage, herbicide usage, number of labor days, quality of soil conservation significantly increased the production value, while slash and burn frequency reduced it; and number of annual crops, number of animal raised, amount of organic fertilizer, and number of labor days reduced farm risks. This indicates that mamar system complies with agriculture environmental soundness principles because it incorporates diverse crops; sound soil conservation nature; various number and kinds of animal raised which results in the provision of manure to mamar and other agro ecosystems; and the limitation of slash and burn. However, the use of inorganic herbicides needs to be controlled.

Keywords: Mamar, Agroforestry, Farm production risk, Indonesia, Timor

Mobina Maktabdaran¹*, Mohammad Hassan Sayyari Zohan², Majid Jami Alahmadi³,

Golam Reza Zamani³

¹MSc Student, Department of Ecology, Faculty of Agriculture, University of Birjand, Iran

²Associate Professor, Department of Soil Science and Engineering, Faculty of Agriculture, University of Birjand, Iran

³Associate Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Birjand, Iran

Abstract

Saffron (Crocus sativus L.) is one of the most important exports products in Iran that proper concentration of nutrients is particularly important in the development and production. Since saffron is reproduced by corm, so always been considered production of replacement corms through appropriate nutrition; and concentration of element in corm in specific stage of growth and development has high correlation with plant yield. Therefore, this study was conducted to determine nutrients concentration in corm of saffron in perennial fields of Qaen and Nehbandan in South Khorasan in 2015. The information of two regions (Qaen and Nehbandan) were collected from 3, 5 and 7 years-old fields. Then, three fields with at least 500 m² under cultivation selected for each age of 3, 5 and 7 years fields and 3 plots from each field and one corm sample from each plot were selected. The concentration of phosphorus (P), potassium (K), nitrogen (N) and iron in corm of saffron were analyzed based on standard laboratory methods. Results revealed that there were significant differences in N, P, K and iron concentrations of saffron corm (P≤0.01), but the effects were distinct for corm number in different weight groups. The present study showed that in saffron fields, N, P, K and iron concentration in corm were the most effective parameters for saffron yield increment, which regression correlations showed yield increment compeer to the change in these indices. Investigation of nutrients concentration of the corm on saffron yield shows the important role of such elements in yield. Therefore, nutrient concentration in saffron corms affect the plant nutrition, promoting growth and yield of crop. Generally, corm selection with optimum weight for sowing and proper use of nutrients, especially nitrogen, phosphorus, potassium and iron, as well as their correct balance in soil, can be effective in yield increment and stability of soil fertility.

Keywords: Corm, Phosphorous, Potassium, Iron, Yield of saffron

Turhadi Turhadi¹*, Hamim Hamim¹, Munif Ghulamahdi², Miftahudin Miftahudin¹

¹Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Kampus IPB Darmaga, 16680 Bogor, Indonesia

²Department of Agronomy and Horticulture, Faculty of Agriculture, Bogor Agricultural University, Kampus IPB Darmaga, 16680 Bogor, Indonesia

Abstract

The acid soil area covers major topics land where Iron (Fe) toxicity is one of a limiting factor for rice production which can be overcome by planting the tolerant variety. The information of morpho-physiological characters and the genetic variation of tolerant genotypes is very important. Here we study the variation of root and shoot growth as well as physiological responses to iron toxicity between ten rice genotypes under hydroponic conditions with agar addition. Growth parameters, leaf bronzing score, Fe content in the shoot and root plaques, total chlorophyll, carotenoids, and malondialdehyde (MDA) were observed in this study. Based on morpho-physiological data related to iron toxicity, ten rice genotypes were clustered into three groups which the best performance genotypes were Pokkali and Hawara Bunar. Leaf bronzing score showed correlated with Fe content in the shoot, but tolerant and sensitive genotypes could be differentiate based on this character because it showed non significant Fe content between those two groups. Our study found that the pattern in morpho-physiological characters variation could be useful for selection of desirable genotypes for Fe tolerant rice.

Keywords: Iron toxicity, Leaf bronzing score, MDA, Morpho-physiological characters, Tolerance

Bindu Singh*, Virendra Kumar Singh, Khalid Monowar Alam

Department of Environmental Science, Integral University, Lucknow, UP, India

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Abstract

Eggplant (Solanum melongena) and green chilli (Capsicum annum) are an important vegetables crop grown throughout the year in the India. However, these vegetables crops suffer heavily from the ravages of various insect pests and disease, which reduce not only the yield but also the quality of the fruit. Malathion is part of the widely used insecticides all around the world. The present study emphasizes the effect of various concentrations and exposure periods of an organophosphorus pesticide. Malathion on two very important vegetative crops eggplant (Solanum melongena) green chilli (Capsicum annum). This study was performed at the department of environmental studies, Integral University, Lucknow (U.P.). The effect of Malathion on growth of the two vegetable crops was observed under greenhouse condition. Sampling of eggplant and green chilli were grown in assorted sets with 2, 4 & 6 ml/L foliar treatment of Malathion. Two sets of control plants were grown without Malathion treatment. They were harvested after 7 & 14 days. Experimental observation revealed that low concentration of Malathion had synergistic effect while higher level had an adverse effect on growth of plant and soil properties. This study infers that whereas lower levels are beneficial. Higher level of pesticides should be avoided and special care be taken to prevent their entry in the food chain.

Keywords: Malathion, Pesticides, Environmental pollution, Vegetative crops

Mónica Galindo¹, Darwin Rueda¹ ⃰, Pedro Romero¹, María Medina¹, Manjunatha Bangeppagari^1,2, Venkata Subba Reddy Gangireddygari³, Sikandar I. Mulla⁴

¹Department of Life Sciences, Universidad de las Fuerzas Armadas-ESPE, Sangolquí, Ecuador, South América

²Center for Biofluid & Biomimic Research, Pohang University of Science and Technology, Pohang, South Korea

³College of Agriculture & Environmental Sciences, Department of Agriculture & Animal Health, Florida Science Campus, Corner Christiaan De Wet and Pioneer Avenue, Florida, University of South Africa, Johannesburg, Gauteng, South Africa

⁴Division of Biotechnology, Chonbuk National University, Iksan, Republic of Korea

Abstract

Potato is the fourth most consumed food product around the world and one of the most produced crops worldwide, due to its nutritional and culinary properties. This crop is affected by certain pests such as phytopathogenic fungi, which frequently attack roots and tubers, extracting their nutrients and decreasing their yield. Consequently, our research is focused in finding an environmentally friendly alternative to improve the nutrition of the crop and prevent the attack of pathogens that exist mostly in soils with poor conditions. One of the ways to reduce the use of fertilizers and pesticides, is the application of beneficial microorganisms, among them fungi (AMF) and Trichoderma harzianum. fungi create symbiosis with the plant and improve mineral absorption with a significant efficiency. Trichoderma harzianum is an effective biological control agent, it induces the defensive response of plants and stimulates plant growing. This investigation evaluated the interaction between Arbuscular mycorrhizal fungi (AMF) and Trichoderma harzianum the development and nutrition of potato plants (Solanum phureja), showing increasing leaf area, biomass (total, aerial and root) and root length in plants inoculated with each of the microorganisms compared to the plants without inoculation (control treatments) and chemical fertilizer applied plants. Namely, the presence of AMF, Trichoderma harzianum and the variation of concentrations of peat at 30% and 50% improved the development, growth and nutrition of potato plants.

Keywords: Solanum phureja, Trichoderma harzianum, Arbuscular mycorrhizal fungi, Peat