Category Archives: b_original_articles

Original Articles

DIFFERENT PATHOGENICITY ASSAYS TO EVALUATE VIRULENCE OF PSEUDOMONAS SYRINGAE PV.PISI ON COMMERCIAL PEA (PISUM SATIVUM L.) VARIETIES

Muhammad Usman Raja1*, Muhammad Waseem Ali1, Farid Asif Shaheen2

1Department of Plant Pathology, 2Department of Entomology,

PMAS-Arid Agriculture University Rawalpindi, Pakistan


ABSTRACT

 

Pea (Pisumsativum L) is a major legume grown in Pakistan which used both as fresh vegetable and canned food. However, pea production in Pakistan is under stress due to various abiotic and biotic factors. Among biotic, the most destructive is bacterial blight caused by Pseudomonassyringae pv pisi(Ppi). Therefore, in present study different pathogenicity assays were performed to observe the innate immunity present in different commercial pea cultivars against Ppi isolated from pea seeds. The results of pathogenicity assays showed that leaf detach method was best method for symptom development as it took 2 days in comparison to foliar injection and soil drenching methods where incubation period prolonged for 6-8 days respectively. Pea varieties viz. DMR-4, DMR-7 Green forest, Rondo and Dasan showed susceptibility against Ppi isolates in all three methods of inoculations. Commercially growing pea germplasm is not showing resistance against Ppi which considered a looming threat to pea productions all over the world. Therefore, an extensive screening of commercial pea germplasm against local bacterial isolates needed to be performed to avoid future crop failure. 

Keywords: Pea, Pisumsativum L, Pseudomonas syringaepvpisi, Bacterial blight

SEASONAL HONEY POLLEN COMPOSITION IN THE SOUDANO-GUINEAN HIGHLAND ZONE OF CAMEROON

D. Dongock Nguemo 1*, J. Tchoumboue 2 and E. Youmbi 3

1Department of Biological Sciences, Faculty of Sciences, University of Ngaoundere,Ngaoundere- Cameroon

2Department of Zootechny, Faculty of Agronomy and Agricultura Sciences, University of Dschang, Cameroon

3Department of Plant Biology & Physiology, Faculty of Sciences, University of Yaounde I. Yaounde, Cameroon

 

 ABSTRACT 

 

In order to assess the influence of seasons on the exploitation of mellliferous plants by Apis mellifera adansonii, seasonal pollen spectra of 104 honey samples collected between  September 2010 and March 2011  in the Highlands area of west Cameroon (LN 5°21.459–5°35.449 and LE 10°04.729–10°26.249) were analysed using melissopalynology methods. The aim was to evaluate the influence of seasons on the exploitation of melliferous plants by A. mellifera adansonii. The spectrum of bee-plants exploited during the rainy season was large and diversified compared to the dry season. Asteraceae and Caesalpiniaceae were the most represented families for the dry and the rainy seasons, respectively. Asteraceae was also the most represented family in the two seasons. The spontaneous Eucalyptus saligna and Terminalia mantaly were much foraged during the two seasons. Flowers of herbs were highly exploited in both the seasons and white colour flowers were frequently foraged. Honey of rainy season was more diversified with four distinguished colours. Predominant and important minor was significantly higher in rainy season compared to the dry season. The frequency of pollen in honey varied considerably with seasons and availability of blooming vegetation exploited by honeybees. Irrespective of vegetation diversity with seasonal variation, honeybee visits were very specific to some plants species.

Keywords: melliferous plants, season, biological characteristic, honey colour, Cameroon

IMPACT OF SOWING TIME AND SEEDING DENSITY ON GRAIN YIELD OF WHEAT VARIETY GOMAL-08

Umer Farooq1, Ejaz Ahmed Khan1, Abdul Aziz Khakwani1Saeed Ahmed2, Nazeer Ahmed*3 and Gohar Zaman4

1Department of Agronomy, Faculty of Agriculture, Gomal University, Dera Ismail Khan-Pakistan

2State University of Londrina Centre of Agriculture Sciences Londrina, Parana (PR) Brazil

3Key Laboratory of Applied Entomology, Northwest A&F University. Yangling, China

4Department of Environmental Science COMSATS University, Vehari campus Multan, Pakistan

 

ABSTRACT

 

The research was performed, at Agronomic Research Area, Faculty of Agriculture, Gomal University Dera Ismail Khan, to assess the impact of sowing time and seeding density on grain yield of wheat variety Gomal-08. Treatments included three dates of sowing as D1 (Oct-25), D2 (Nov-20), D3 (Dec-15) and three seeding rates (kg ha-1) of S1-100, S2-120, S3-150. Almost all yield parameters showed significant variability among treatments means. Maximum grain spike-1(g), number of fertile tillers m-2, spike length (cm), economic yield (t ha-1) and thousand grain weight (g) were recorded in D1(Oct-25) and D2 (Nov-20) with S1 and S2 as compared to late planted wheat with higher seed rate applied.

 

Keywords: Wheat, Plant Density, Sowing Date, Production

FIRST RECORD OF THREE SPECIES OF GENUS POLISTES (VESPIDAE: HYMEMOPTERA) FROM DISTRICT LAYYAH (PUNJAB), PAKISTAN

Imran Bodlah1*, Hamza Ali1, Muhammad Akram2, Muhammad Naeem1, Ata ul Mohsin1 and Muhammad Asif Aziz1

1 Laboratory of Biosystematics, Department of Entomology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan. 2 Plant Protection, Pest warning and Quality control of Pesticides, Chakwal, Pakistan

 ABSTRACT 

Three species of Genus Polistes (Vespidae: Hymenoptera) are reported for the first time from different localities of district Layyah (Punjab), Pakistan. Main identification characters, distribution and micrographs have been provided for future field and research identification.

 

Keywords: Hymenoptera, Vespidae, Genus Polistes, Layyah, Punjab

PHYTOTOXICITY OF DIFFERENT AQUEOUS EXTRACT CONCENTRATIONS OF MESQUITE (PROSOPIS JULIFLORA) ON GERMINATION AND SEEDLING GROWTH OF WHEAT

Rafaqat Hussain Shah1*, Mohammad Safdar Baloch1, Muhammad Zubair2Ejaz Ahmad Khan1, Muhammad Ahmad3 and Ghulam Abbas4

1 Department of Agronomy, Faculty of Agriculture, Gomal University, Dera Ismail Khan, Pakistan. 2 The Department of Forestry and Range Management, Faculty of Agricultural Sciences and Technology, Bahauddin Zakariya University, Multan, Pakistan, 3Agrivcultural Training Institute, Karor Lal Eason, Layyah, Pakistan, 4Adaptive Research Farm, Karor Lal Eason, Layyah, Pakistan

 

 ABSTRACT 

 

The research trial was conducted under laboratory conditions at Department of Agronomy, Faculty of Agriculture, Gomal University, Dera Ismail Khan, Pakistan in order to study phytotoxic effects of mesquite (Prosopis juliflora) aqueous extract taken from its leaves, stem and root on germination and seedling growth of wheat. There were five treatments viz. 100g, 200g, 300g, and 400g aqueous leaves, stem and root extract of mesquite including tap water as control. It was evident from the results that by using different concentrations of aqueous extract of mesquite, seed germination and seedling growth of wheat were inhibited. The maximum reduction in germination and seedling growth was noted by applying higher concentration (400g) of aqueous extracts of mesquite. Hence, it is concluded that seed germination and seedling growth of wheat is inhibited due to presence of water soluble allelochemicals in aqueous leaves, stem and root extracts of mesquite at 400 g for 72 hours. These allelochemicals have negative effects on growth and development of wheat. It is, therefore, recommended not to cultivate wheat crop near the mesquite.

 

Keywords: Phytotoxicity, Prosopis juliflora, extract, germination, seedling vigor, wheat

EFFECT OF DIFFERENT BRASSICA VEGETABLES ON BIOLOGY AND DEMOGRAPHIC PARAMETERS OF BREVICORYNE BRASSICAE (HOMOPTERA: APHIDIDAE) UNDER LABORATORY CONDITIONS

Muhammad Asif Aziz1, Muhammad Irfan1, Imran Bodlah1 and Muhammad Hanif2

1Department of Entomology, 2Department of  Mathematics and Statistics

Pir Mehr Ali Shah, Arid Agriculture University, Rawalpindi 

ABSTRACT 

Biological and demographic parameters of cabbage aphid, Brevicoryne brassicae (L.) were studied on four different Brassica hosts; Cabbage, Broccoli, China cabbage, and Knol khol under laboratory conditions. Age-stage, two-sex life table studies showed significantly higher intrinsic rate of increase (rm) and finite rate of increase (λ)  on China cabbage followed by Broccoli and Cabbage. Performance of cabbage aphids remained comparatively low on Knol khol in terms of intrinsic rate of increase (rm), finite rate of increase (λ) and net reproductive rate. Total duration of immature stages was significantly longer on Knol khol than Cabbage and China cabbage, however, adult pre-reproductive period showed non-significant variation. Reproductive period, adult longevity and female fecundity upon all the studied plant species were significantly different. The minimum fecundity and adult longevity were observed on Knol khol. The probability of surviving nymph to adult female was 0.25, 0.35, 0.50 and 0.60 on Cabbage, Knol khol, Broccoli and China cabbage, respectively. The gross reproductive rate, life expectancy and age stage reproductive values were also minimal on Knol khol. On the basis of present life table studies, China cabbage remained the most susceptible host followed by both Broccoli and Cabbage while Knol khol proved to be the most unsuitable host. Present studies confirm Knol khol as comparatively more resistant against cabbage aphid and suitable to be cultivated as important vegetable crop in Pothwar region of Punjab.

Keywords: Brevicoryne brassicae, Brassica, life table, development, fecundity, age-stage two-sex

ANTIHYPERLIPIDEMIC EFFICACY OF CINNAMON IN ALBINO RATS

Zahid Iqbal1*, Taseer Ashraf1, Aamir Ali Khan2, Riaz Hussain3, Mohiuddin Mudassar4

1Department of Pharmacology, Al-Nafees Medical College, Isra University, Islamabad, Pakistan, 2Department of Pathology, Bakhtawar Amin Medical and Dental College, Multan, Pakistan, 3Department of Pathobiology, University College of Veterinary and Animal Sciences, The Islamia University, Bahawalpur, Pakistan, 4Department of Pathology, Al-Nafees Medical College, Isra University, Islamabad, Pakistan

 ABSTRACT 

The objective of this study was to assess the antihyperlipidemic efficacy of cinnamon powder in albino rats. For this purpose 180 adult albino rats (average weight 210 ± 11 grams) were purchased and divided into six groups. In rats blood lipid profile was raised using cholesterol @ 400mg/Kg body weight of rat which was mixed into rat feed for first 15 days of the study. Cinnamon bark powder equivalent to 1gm/kg, 2gm/kg, 4gm/kg and 6gm/kg was administered to the rats of treatment groups 15-60 days. Treatment control group was given Simvastatin at the dose rate of 0.6mg/Kg body weight. Blood samples were collected at 0, 15, 30, 45 and 60 days in sterilized gel tubes by direct heart puncturing. Serum was separated and analyzed for lipid profile parameters using reagent kits. Findings of present study revealed that various doses of Cinnamomum cassiae powder improved the serum lipid profile in albino rats by reducing Total Lipids, Total Cholesterol, Triglycerides and LDL cholesterol and increasing HDL cholesterol levels in cinnamon treated groups. Furthermore, the most significant effect was shown by 6 mg/kg dose level. From the results of the present study, it was concluded that Cinnamon powder has curative effect against hyperlipidemia.

 

Keywords: Cinnamon, Antihyperlipidemic efficacy, Albino rats, Lipid profile, Cholesterol

ECONOMIC COMPARISON OF DIRECT SEEDED AND TRANSPLANTED RICE: EVIDENCES FROM ADAPTIVE RESEARCH AREA OF PUNJAB PAKISTAN

Muhammad Younas1*, Momna Abdul Rehman2,

Abdi Hussain3, Liaqat Ali4 and Masood Qadir Waqar5

1Senior Subject Matter Specialist (FM&FE), Adaptive Research Farm, Sheikhupura,

2Assisant Research Officer (FM&FE), Adaptive Research Farm, Sheikhupura,

3Senior Scientific Officer, Social Sciences Research Institute, NARC, Islamabad,

4Assistant Research Officer, Adaptive Research Farm, Sheikhupura,

5Director of Agriculture, Adaptive Research Punjab, Lahore

 ABSTRACT 

The study was conducted in three of the four districts of Adaptive Research Zone, Sheikhupura; which contains Sheikhupura, Lahore and Kasur districts for economic comparison of direct seeded (DSR) and transplanted super basmati rice during Kharif-2014. Average yield, cost of production, net economic benefits and benefit cost ratio for DSR and transplanted rice was calculated. Extent of area under DSR technology was also determined. Farmers growing both DSR and transplanted rice were selected using purposive sampling technique and sample size was 36. Data was collected by face to face interview by using a well-structured questionnaire having personal, farm related and cost variables. The results revealed that DSR technology was adopted on 22.8% of rice area on surveyed farms. Average paddy yield, total cost of production, net economic benefits per hectare and BCR for direct seeded rice were 3.09, Rs.112047, Rs.15014, 1.11 and for transplanted the figures were 3.19, Rs. 134882, Rs.-8433, 0.95 respectively. Thus, in the year 2014, farmers gained profit from practicing DSR technology and born loss from transplanted rice crop. It was found that farmers were growing DSR through seven different sowing methods and using different seed rates. Moreover, weeds infestation is a major problem in DSR technology that can be overcome through a combination of cultural as well chemical control methods. It was concluded that DSR is a promising technology subject to weeds management. Better coordination between research, extension and farming community can bring fruitful results with respect to adoption of DSR technology.

Keywords: DSR, Transplanted rice, Net Benefits, BCR

RESPONSE OF SWEET BELL PEPPER TO MORINGA LEAF EXTRACT AND ORGANO-BIO DEGRADABLE FERTILIZER

Oluwagbenga Dunsin and Theo Okiemute Odeghe
Department of Crop & Soil Sciences, Landmark University, Omu-Aran, Kwara-State. Nigeria
 
ABSTRACT
 
An experiment was carried out at the Teaching and Research Farm of Landmark University Omu-Aran, Nigeria in the Savannah ecosystem of Nigeria from November to March for evaluating the response of sweet bell pepper to Moringa Leaf Extract (MLE) and Organo Bio Degradable fertilizer (OBD+). The treatments included four levels of Organo-Bio Degradable fertilizer (0g, 20g, 40g and 60g) Moringa Leaf Extract at ratio 1:32 (v/v) was sprayed directly at the plant at 1 and 2 weeks after transplanting respectively and replicated thrice in a Completely Randomized Design (CRD).The results showed that plant height, number of leaves, fruit weight and yield of sweet bell pepper were significantly (P≤0.05) influenced by the application of  Moringa Leaf Extract and Organo-Bio Degradable fertilizer (OBD+).
Keywords: Sweet bell pepper; Moringa Leaf Extract; Organo-biofertilizer (OBD+)

CONTROL OF VARROA DESTRUCTOR ANDERSON AND TRUEMAN (ACARI: VARROIDAE) ON APIS MELLIFERA LINGUISTICA BY USING THYMOL AND FORMIC ACID IN POTHWAR REGION OF PUNJAB, PAKISTAN

Muhammad Asif Aziz*, Muhammad Azeem, Muhammad Sheeraz Ahmed, Farva Siddique, Muhammad Jamal
Department of Entomology, Pir Mehr Ali Shah, Arid Agriculture University Rawalpindi, Pakistan
 
ABSTRACT
 
Varroa destructor Anderson and Trueman (Acari: Varroidae) is the most devastating pest for beekeeping in Pakistan.Two acaricides i.e., thymol (powdered form) and formic acid were tested on Apis mellifera linguistica coloniesfor the management of Varroa mites in modified German and traditional Langstroth beehives. At first thymol was applied in two doses (2 and 4 gm) at weekly interval and the data were taken for four weeks. Efficacy of thymol 4 gm was better on bee colonies as compared to those treated with 2gm thymol. In modified German bee hives more mortality of mites was recorded due to thymol treatment as compared to traditional bee hives. In the next step both thymol and formic acid were compared for their effectiveness against Varroa mite. The results indicated that, formic acid (30ml/hive) had better efficacy than thymol 4gm. Both the acaricides were more effective on modified German beehives as compared to traditional Langstroth bee hives. No queens were lost, and no adult honeybee mortality was observed in any of the colonies during the experiment.  
Keywords: Apis mellifera, Varroa destructor, thymol, formic acid