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Volume 4, Issue 3      July - September, 2016

DIFFERENT PATHOGENICITY ASSAYS TO EVALUATE VIRULENCE OF PSEUDOMONAS SYRINGAE PV.PISI ON COMMERCIAL PEA (PISUM SATIVUM L.) VARIETIES
 

Muhammad Usman Raja1*, Muhammad Waseem Ali1, Farid Asif Shaheen2

1Department of Plant Pathology, 2Department of Entomology,

PMAS-Arid Agriculture University Rawalpindi, Pakistan


ABSTRACT

 

Pea (Pisumsativum L) is a major legume grown in Pakistan which used both as fresh vegetable and canned food. However, pea production in Pakistan is under stress due to various abiotic and biotic factors. Among biotic, the most destructive is bacterial blight caused by Pseudomonassyringae pv pisi(Ppi). Therefore, in present study different pathogenicity assays were performed to observe the innate immunity present in different commercial pea cultivars against Ppi isolated from pea seeds. The results of pathogenicity assays showed that leaf detach method was best method for symptom development as it took 2 days in comparison to foliar injection and soil drenching methods where incubation period prolonged for 6-8 days respectively. Pea varieties viz. DMR-4, DMR-7 Green forest, Rondo and Dasan showed susceptibility against Ppi isolates in all three methods of inoculations. Commercially growing pea germplasm is not showing resistance against Ppi which considered a looming threat to pea productions all over the world. Therefore, an extensive screening of commercial pea germplasm against local bacterial isolates needed to be performed to avoid future crop failure. 

Keywords: Pea, Pisumsativum L, Pseudomonas syringaepvpisi, Bacterial blight

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