Beenish Zia Butt*, Sumera Javad, Saba Zia

Lahore College for Women University, Lahore, Pakistan

Abstract

Recently the biosynthesis of nanoparticles using plant extracts has drawn the attention of researchers. The aim of the present study is to investigate the potential of Catharanthus roseus for the biological synthesis of iron nanoparticles and to evaluate the antifungal efficacy of the synthesized particles against the selected fungal strains. Biosynthesis of iron nanoparticles was performed through the n-hexane leaf extract of Catharanthus roseus. The structural properties of the synthesized nanoparticles (concentrations, 10%, 30%) were further investigated through UV-visible spectroscopy; particle size analyzer (PSA), scanning electron microscopy (SEM) and fourier transform infrared spectroscopy (FTIR). Results of the UV-Vis spectroscopy of the synthesized iron nanoparticles showed the absorption spectra of iron nanoparticles (FeNPs), prominent peak at 260 nm corresponding to the absorption of iron nanoparticles was obtained. Particles size analyzer revealed the average size of the iron nanoparticles that was calculated as 108 and 266 nm. Phenolics (OH group at 3350 cm^-1) were observed as main bioactive phytochemical of the plant extract that acted as capping agent in iron nanoparticles synthesis. Surface morphology using SEM revealed the aggregates of irregular shaped iron nanoparticles. The antifungal activity of these biosynthesized FeNPs against Aspergillus nidulans and Aspergillus terreus was also evaluated, nanoparticles showed high activity against A. terreus. This study concludes that the biosynthesis of iron nanoparticles is a safe and economical approach.

Keywords: Nanoparticles, Green synthesis, Catharanthus roseus, n-Hexane, capping agent, Economical approach.

N’dri Aya Lydie^1,2*, Ahouchi Simon Valencourt¹, Fofana Nambate¹, Yapi Jean Noel¹

¹Laboratory of Biology and Animal Cytology, Research and Training Unit of Natural Sciences-University of Nangui Abrogoua, 02 BP 801 Abidjan 02, Côte d’Ivoire.

²Laboratory of Genetic and Bioresources Improvement, Research and Training Unity- University of Nangui Abrogoua, 02 BP 801 Abidjan 02, Côte d’Ivoire.

Abstract

In Côte d’Ivoire, the local hen breeding constitutes nearly 70% of national poultry livestock in Côte d’Ivoire. Local hens of Côte d’Ivoire in general, are less known by scientific community because it is almost fewer characterized. In this context, it is necessary to work with the aim of improving local hens’ productivity in order to contribute at food self-sufficiency. Therefore, the present study was carried out to assess the shelf life and placement of the egg before brooding.240 eggs from 82 local hens aged 36 weeks were divided in 6 batches of 40 eggs each. At first 3 batches were stored during 3 days, 5 days and 10 days respectively for batch 1, batch 2 and batch 3. Secondarily, the 3 others batches were stored during 5 days using 3 breeding positions namely vertical position with small end up, vertical position with the small end down and the horizontal position. Influences of storage duration on eggs weight and position of eggs on hatchability characteristics were evaluated.  The study showed that the positioning of the eggs significantly influenced hatchability and the horizontal storage position gave the best results compared to the two other positions. The average fertility of the local eggs recorded in this study showed out that long-term stored eggs presented lower hatching average. For the local hens, a storage of 5 days maximum before incubation of the eggs is advisable. The positioning and storage time significantly influenced the weight and hatchability of local eggs.

Keywords: Storage, Egg position, Hatchability, Local hens, Cote d’Ivoire

Thura Alyasiri*¹, Salah Alchalabi², Ithar AlMayaly¹

¹Department of Biology, College of Science, Baghdad University, Iraq

²Biotechnology Research Center, Al-Nahrain University, Iraq

Abstract

Lead is considered one of the wide spread environmental pollutants in Iraq. Nowadays, some types of algae can be the solution by utilizing them as natural medications to cure many diseases. This scientific article is intended to examine spirulina action as an antioxidant to cure lead acetate induced injury in rats. The total numbers of rats used were (48), the rats were divided into eight groups, (42 rats) represented the treated group and 6 rats represented the control group. 36 Rats of treated groups were injected initially with different concentrations of lead acetate; while the rest 6 rats were only fed with Spirulina. All our samples were examined by biochemical, hematological and immunohistological methods. Our experiments proved that Spirulina had an antioxidant action which can support the body defense system. Malondialdehyde (MAD), Superoxide Dismutase (SOD) and Catalase (CAT) were increased in lead injected group; while they decreased in the spirulina fed group. There was a significant enhancement in lipid profile values of treated group that were fed spirulina. In addition, it noticed that the values of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in rats injected by lead, then fed spirulina were decreased; this decrement was evidence in the histological results. Moreover, the hematological results of rats fed with spirulina appeared that the leukocyte and platelet numbers also decreased; while the erythrocyte, hemoglobin, and hematocrit levels were increased, unlike, rats injected with only lead. The conclusion was that Spirulina ability to do obvious decrement in the poisonous action of lead was done by its scavenger free radical activity and its effective antioxidant activity.

Keywords: In vivo, Antioxidant, Spirulina, Lead toxicity, Hepatoprotective

Noor Asyraf Noor Azman, Nik Ahmad Nizam Nik Malek*, Norfarah Syuhaidah Mohd Noor, Muhammad Arshad Javed

Department of Biotechnology and Medical Engineering, Faculty of Biosciences and Medical Engineering,

Universiti Teknologi Malaysia, 81310 UTM, Skudai, Johor, Malaysia

Abstract

There is great interest in using sustainable fertilizer to enhance medicinal plant quality such as Centella asiatica, which is known for its various medicinal properties. Here, we examined the performance of NPK-Organo-Zeolite (NPKOZ) as a controlled release fertilizer for the growth of C. asiatica. Natural zeolite that has been modified with surfactant hexadecyltrimethyl ammonium (HDTMA) and addition with ammonium (N), phosphate (P) and potassium (K) was characterized with X-ray diffraction and Fourier transform infrared spectroscopy. In a leaching study, it was found the NPKOZ showed fewer losses of nutrients. For the plant growth study, a comparison was made with eight different treatments including chemical fertilizers monoammonium phosphate and monopotassium phosphate for 70 days. Morphological (number of flowers, branches and leaves, specific leaf area and plant biomass) and biochemical growth parameters (N, P, K and chlorophyll contents) of the plant were analysed. Second cultivation was also conducted to compare potted, hydroponic and verticulture systems with or without NPKOZ. The NPKOZs treatments resulted in the highest number of leaves, branches and flowers among all treatments. In addition, the chlorophyll contents were also the highest with NPKOZs application. The NPK contents in the C. asiatica plants were comparable with that of chemical fertilizers tested. This study showed that NPKOZ fertilizer with verticulture system is a suitable sustainable controlled release fertilizer for the improvement of the growth of C. asiatica.

Keywords: Centella asiatica, Surfactant modified zeolite, Controlled release fertilizer

Rabiya Ikram, Basharat Ali*

Department of Microbiology and Molecular Genetics, University of the Punjab, Lahore, Pakistan

Abstract

The main objective of this study was to evaluate the effect of cadmium resistant bacteria to enhance the growth of black gram (Vigna mungo) under cadmium (CdCl₂) stress. For this purpose, plant growth promoting rhizobacteria (PGPR) and strains of rhizobia were isolated from the rhizosphere of different plants. 16S rRNA gene sequencing of bacteria confirmed the presence of the strains of Bacillus cereus, B. thuringiensis, Pseudomonas fluorescens and Rhizobium. L-tryptophan dependent auxin production was determined colorimetrically by growing strains in Nutrient broth. B. Cereus HG, B. thuringiensis JAF and Rhizobium sp. IBA showed highest levels of auxin production that ranges from 140 to 146 µg ml^-I. For pot trials, soil was amended with 50, 100 and 200 mg kg^-1. In single bacterial inoculations, significant shoot length response was up to 65% and 39% with P. fluorescens WN-1 and B. cereus CF at 200 mg kg^-I, over respective CdCl₂ treated control. Similarly, for fresh biomass, 2.2 and 1.3 fold increases were shown by B. cereus CF at 100 and 200 mg kg^-I CdCl₂ stress, respectively. For mixed culture of PGPR, combination MC-3 (CF-WN-1, A-2) significantly promoted shoot length (1 fold) at 50 mg kg^-I CdCl₂. In case of mixed culture of PGPR and rhizobia, up to 1 fold increases were observed for shoot length, root growth and fresh biomass with MCR-1 (IBA, HG, BS-1) at 100 mg kg^-I CdCl₂. Finally, it can be concluded that strains isolated from the rhizosphere of different pants showed promising results for plant growth promotion in cadmium amended soils.

Keywords: Bacterial auxin, Cadmium stress, PGPR, Growth promotion, Phytoremediation, Vigna mungo

Muhammad Abbas*, Muhammad Irfan, Javaid Ahmed Shah, Muhammad Yousuf Memon

Soil and Environmental Sciences Division, Nuclear Institute of Agriculture (NIA), Tando Jam, Sindh, Pakistan