1Senior Scientific Officer, On-Farm Research Division, Bangladesh Agricultural Research Institute (BARI), Sylhet-3100, Bangladesh and 2Principal Scientific Officer, Regional Wheat Research Station, Bangladesh Agricultural Research Institute (BARI), Gazipur, Bangladesh

 

Soil acidification threatens dry land crop production in the soils of entire Sylhet region in Bangladesh. Our objective was to assess the efficacy of lime doses with agroeconomic performances of wheat varieties to lower soil acidity and increase wheat production in a direct-seeded system. Field trail was carried out at Eastern Surma Kushiara Flood Plain soil (AEZ 20) of Sylhet in the three consecutive years of 2010-11, 2011-12 and 2012-13. Treatments of wheat varieties (Bijoy, Prodip, Sufi, BARI Gom-25 and BARI Gom-26) and lime (0 and 1.5 tha-1) arranged in a split-plot design. Lime was applied in main plots and varieties are distributed randomly in sub plots. Soil was sampled in November 2010 to assess pH and Low pH was evident at the depth of fertilizer placement (0-30 cm). Broadcast lime increased pH up to 6.0 in 2013. The result revealed that most of the yield component viz. spikes m-2, thousand grain weight and grain yield of wheat was significantly influenced by liming. There were variations in lime response among the wheat varieties. Results observed that all the varieties performed well in the liming plot (3.39-4.09 tha-1) and BARI Gom-26 produced the maximum yield (4.09 tha-1) among them compare to non liming one (2.48-2.97 tha-1). Cost benefit analysis indicated that the highest BCR was recorded in BARI Gom-26 in the liming plot that was 24% more compared to non liming plot. However, the index of relative adaptability of wheat was the maximum in BARI Gom-26 and it could be cultivated at acidic soil in Sylhet, Bangladesh.

Keywords: Soil acidity, liming, wheat, economic analysis, relative adaptability index.

1Department of Pathology, Al-Nafees Medical College, Isra University, Islamabad Campus, Islamabad.

2Animal Sciences Divison, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad.

3Department of Pharmacology, Al-Nafees Medical College, Isra University, Islamabad Campus, Islamabad.

4Institute of Microbiology, University of Agriculture, Faisalabad.

 

Based on safety and sound immunological principles for animal vaccines, there arises a need for identifying rational standards for selection of different adjuvant formulations. Montanide ISA206 (W/O/W) was used in this study to prepare the vaccine and was compared with already available paraffin oil and alum adjuvanted hemorrhagic septicemia vaccines. All the three vaccines were prepared according to the standard procedures keeping the antigen amount same in all the three vaccine formulations. The goal of the study was to ascertain immune response in rabbits against several Pasteurella multocida vaccine preparations including Montanide adjuvanted, paraffin oil based and alum-precipitated vaccines. Forty male adult rabbits were divided into four groups namely A, B, C and D and kept in separate cages. Rabbits in groups A, B and C were vaccinated via subcutaneous route with 0.3ml dose of Montanide vaccine, oil based vaccine and alum-precipitated vaccine respectively. A booster dose of 0.3 ml of same preparation was given 15 days post-vaccination. Rabbits in group D were kept unvaccinated as negative control. All rabbits were bled pre-vaccination and then every 7 days post-vaccination for nine weeks and sera were subjected to indirect haemagglutination assay (IHA) and geometric mean titres of anti-Pasteurella multocida antibodies were determined. The mean and standard errors were also determined. Results showed that Montanide ISA206 (W/O/W) adjuvanted vaccine gave higher antibody titres as compared with oil based and alum-precipitated vaccines.

Keywords: Montanide ISA-206; Pasteurella multocida; Hemorrhagic septicemia vaccine; Geometric mean titre; Immune response