Archive
 
1.0
2021CiteScore
 
36th percentile
Powered by  Scopus
Volume 2, Issue 2      April-June, 2014

IMMUNE RESPONSE OF RABBITS TO HEMORRHAGIC SEPTICEMIA VACCINE FORMULATIONS ADJUVANTED WITH MONTANIDE ISA-206, PARAFFIN OIL AND ALUM
 
Mohi-ud-din, Mudassar.*1, Mudasser, Habib. 2, Zahid, Iqbal. 3 Iftikhar, Hussain. 4
1Department of Pathology, Al-Nafees Medical College, Isra University, Islamabad Campus, Islamabad.
2Animal Sciences Divison, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad.
3Department of Pharmacology, Al-Nafees Medical College, Isra University, Islamabad Campus, Islamabad.
4Institute of Microbiology, University of Agriculture, Faisalabad.
 
ABSTRACT
Based on safety and sound immunological principles for animal vaccines, there arises a need for identifying rational standards for selection of different adjuvant formulations. Montanide ISA206 (W/O/W) was used in this study to prepare the vaccine and was compared with already available paraffin oil and alum adjuvanted hemorrhagic septicemia vaccines. All the three vaccines were prepared according to the standard procedures keeping the antigen amount same in all the three vaccine formulations. The goal of the study was to ascertain immune response in rabbits against several Pasteurella multocida vaccine preparations including Montanide adjuvanted, paraffin oil based and alum-precipitated vaccines. Forty male adult rabbits were divided into four groups namely A, B, C and D and kept in separate cages. Rabbits in groups A, B and C were vaccinated via subcutaneous route with 0.3ml dose of Montanide vaccine, oil based vaccine and alum-precipitated vaccine respectively. A booster dose of 0.3 ml of same preparation was given 15 days post-vaccination. Rabbits in group D were kept unvaccinated as negative control. All rabbits were bled pre-vaccination and then every 7 days post-vaccination for nine weeks and sera were subjected to indirect haemagglutination assay (IHA) and geometric mean titres of anti-Pasteurella multocida antibodies were determined. The mean and standard errors were also determined. Results showed that Montanide ISA206 (W/O/W) adjuvanted vaccine gave higher antibody titres as compared with oil based and alum-precipitated vaccines.
Keywords: Montanide ISA-206; Pasteurella multocida; Hemorrhagic septicemia vaccine; Geometric mean titre; Immune response
Download PDF

FacebookTwitterEmailWhatsApp
 
     
 
 
 
Asian Journal of Agriculture and Biology © 2017  
Asian Journal of Agriculture and Biology is licensed under a 
Creative Commons Attribution 3.0 License.