Category Archives: b_original_articles

Original Articles

Relationship between nutrient concentration in saffron corms and saffron yield in perennial fields of South Khorasan province

Mobina Maktabdaran1*, Mohammad Hassan Sayyari Zohan2, Majid Jami Alahmadi3,

Golam Reza Zamani3

1MSc Student, Department of Ecology, Faculty of Agriculture, University of Birjand, Iran

2Associate Professor, Department of Soil Science and Engineering, Faculty of Agriculture, University of Birjand, Iran

3Associate Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Birjand, Iran

Abstract

Saffron (Crocus sativus L.) is one of the most important exports products in Iran that proper concentration of nutrients is particularly important in the development and production. Since saffron is reproduced by corm, so always been considered production of replacement corms through appropriate nutrition; and concentration of element in corm in specific stage of growth and development has high correlation with plant yield. Therefore, this study was conducted to determine nutrients concentration in corm of saffron in perennial fields of Qaen and Nehbandan in South Khorasan in 2015. The information of two regions (Qaen and Nehbandan) were collected from 3, 5 and 7 years-old fields. Then, three fields with at least 500 m2 under cultivation selected for each age of 3, 5 and 7 years fields and 3 plots from each field and one corm sample from each plot were selected. The concentration of phosphorus (P), potassium (K), nitrogen (N) and iron in corm of saffron were analyzed based on standard laboratory methods. Results revealed that there were significant differences in N, P, K and iron concentrations of saffron corm (P≤0.01), but the effects were distinct for corm number in different weight groups. The present study showed that in saffron fields, N, P, K and iron concentration in corm were the most effective parameters for saffron yield increment, which regression correlations showed yield increment compeer to the change in these indices. Investigation of nutrients concentration of the corm on saffron yield shows the important role of such elements in yield. Therefore, nutrient concentration in saffron corms affect the plant nutrition, promoting growth and yield of crop. Generally, corm selection with optimum weight for sowing and proper use of nutrients, especially nitrogen, phosphorus, potassium and iron, as well as their correct balance in soil, can be effective in yield increment and stability of soil fertility.

Keywords: Corm, Phosphorous, Potassium, Iron, Yield of saffron

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Morpho-physiological responses of rice genotypes and its clustering under hydroponic iron toxicity conditions

Turhadi Turhadi1*, Hamim Hamim1, Munif Ghulamahdi2, Miftahudin Miftahudin1

1Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Kampus IPB Darmaga, 16680 Bogor, Indonesia

2Department of Agronomy and Horticulture, Faculty of Agriculture, Bogor Agricultural University, Kampus IPB Darmaga, 16680 Bogor, Indonesia

Abstract

The acid soil area covers major topics land where Iron (Fe) toxicity is one of a limiting factor for rice production which can be overcome by planting the tolerant variety. The information of morpho-physiological characters and the genetic variation of tolerant genotypes is very important. Here we study the variation of root and shoot growth as well as physiological responses to iron toxicity between ten rice genotypes under hydroponic conditions with agar addition. Growth parameters, leaf bronzing score, Fe content in the shoot and root plaques, total chlorophyll, carotenoids, and malondialdehyde (MDA) were observed in this study. Based on morpho-physiological data related to iron toxicity, ten rice genotypes were clustered into three groups which the best performance genotypes were Pokkali and Hawara Bunar. Leaf bronzing score showed correlated with Fe content in the shoot, but tolerant and sensitive genotypes could be differentiate based on this character because it showed non significant Fe content between those two groups. Our study found that the pattern in morpho-physiological characters variation could be useful for selection of desirable genotypes for Fe tolerant rice.

Keywords: Iron toxicity, Leaf bronzing score, MDA, Morpho-physiological characters, Tolerance

The impact of organophosphorus pesticide on Solanum melongena, Capsicum annum and Soil

Bindu Singh*, Virendra Kumar Singh, Khalid Monowar Alam

Department of Environmental Science, Integral University, Lucknow, UP, India

.

Abstract

Eggplant (Solanum melongena) and green chilli (Capsicum annum) are an important vegetables crop grown throughout the year in the India. However, these vegetables crops suffer heavily from the ravages of various insect pests and disease, which reduce not only the yield but also the quality of the fruit. Malathion is part of the widely used insecticides all around the world. The present study emphasizes the effect of various concentrations and exposure periods of an organophosphorus pesticide. Malathion on two very important vegetative crops eggplant (Solanum melongena) green chilli (Capsicum annum). This study was performed at the department of environmental studies, Integral University, Lucknow (U.P.). The effect of Malathion on growth of the two vegetable crops was observed under greenhouse condition. Sampling of eggplant and green chilli were grown in assorted sets with 2, 4 & 6 ml/L foliar treatment of Malathion. Two sets of control plants were grown without Malathion treatment. They were harvested after 7 & 14 days. Experimental observation revealed that low concentration of Malathion had synergistic effect while higher level had an adverse effect on growth of plant and soil properties. This study infers that whereas lower levels are beneficial. Higher level of pesticides should be avoided and special care be taken to prevent their entry in the food chain.

Keywords: Malathion, Pesticides, Environmental pollution, Vegetative crops

Evaluation of the interaction of arbuscular mycorrhizal fungi and Trichoderma harzianum in the development and nutrition of potato plants (Solanum phureja)

Mónica Galindo1, Darwin Rueda1 , Pedro Romero1, María Medina1, Manjunatha Bangeppagari1,2, Venkata Subba Reddy Gangireddygari3, Sikandar I. Mulla4

1Department of Life Sciences, Universidad de las Fuerzas Armadas-ESPE, Sangolquí, Ecuador, South América

2Center for Biofluid & Biomimic Research, Pohang University of Science and Technology, Pohang, South Korea

3College of Agriculture & Environmental Sciences, Department of Agriculture & Animal Health, Florida Science Campus, Corner Christiaan De Wet and Pioneer Avenue, Florida, University of South Africa, Johannesburg, Gauteng, South Africa

4Division of Biotechnology, Chonbuk National University, Iksan, Republic of Korea

 

Abstract

Potato is the fourth most consumed food product around the world and one of the most produced crops worldwide, due to its nutritional and culinary properties. This crop is affected by certain pests such as phytopathogenic fungi, which frequently attack roots and tubers, extracting their nutrients and decreasing their yield. Consequently, our research is focused in finding an environmentally friendly alternative to improve the nutrition of the crop and prevent the attack of pathogens that exist mostly in soils with poor conditions. One of the ways to reduce the use of fertilizers and pesticides, is the application of beneficial microorganisms, among them fungi (AMF) and Trichoderma harzianum. fungi create symbiosis with the plant and improve mineral absorption with a significant efficiency. Trichoderma harzianum is an effective biological control agent, it induces the defensive response of plants and stimulates plant growing. This investigation evaluated the interaction between Arbuscular mycorrhizal fungi (AMF) and Trichoderma harzianum the development and nutrition of potato plants (Solanum phureja), showing increasing leaf area, biomass (total, aerial and root) and root length in plants inoculated with each of the microorganisms compared to the plants without inoculation (control treatments) and chemical fertilizer applied plants. Namely, the presence of AMF, Trichoderma harzianum and the variation of concentrations of peat at 30% and 50% improved the development, growth and nutrition of potato plants.

Keywords: Solanum phureja, Trichoderma harzianum, Arbuscular mycorrhizal fungi, Peat

Genetic parameters for body weight and egg production traits in Taiwan native chicken homozygous for the heat shock protein 70 gene

Kang Shann-Ren1, Lin Cheng-Yung1, Cheng Yu-Shin2, Lin Der-Yuh2, Huang Tsung-Ping3, Hung Kuo-Hsiang4, Liang Hsiao-Mei1*

1Kaohsiung Animal Propagation Station, Livestock Research Institute, Council of Agriculture, Pingtung 91247, Taiwan

2Livestock Research Institute, Council of Agriculture, Tainan, 71246, Taiwan

3Animal Propagation Department, Taiwan Sugar Corporation, Tainan 70176, Taiwan

4Graduate Institute of Bioresources, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan

 

Abstract

Taiwan native chicken strain (Taishu No. 7) carrying homozygous gene for heat shock protein 70 (HSP70) was evaluated for heritability, genetic and phenotypic correlations for body weight at birth (BW0), body weight at 16 weeks of age (BW16), egg weight at first egg (FEW), body weight at first egg (BWFE), age at first egg (AFE) and number of eggs laid up to 40 weeks of age (EN40) in female chickens. The multivariate animal model using the residual maximum likelihood procedure was applied. The estimations realized over three generations and the pedigree file was composed of 141 individuals. Heritability estimates of BW0, BW16, FEW, AFE, BWFE, and EN40 were 0.306, 0.763, 0.071, 0.284, 0.681, and 0.200, respectively. The body weight traits showed that the BW16 had the negative genetic correlations with BW0, AFE, and EN40 (rg = –0.061; rg = –0.242; rg = –0.087), but had the positive genetic correlations with FEW and BWFE (rg =0.419; rg =0.945). The egg production traits showed that the EN40 had the negative genetic correlations with BW16 and AFE (rg= –0.087; rg = –0.195), but had the positive genetic correlation with BW0, FEW, and BWFE (rg = 0.336; rg = 0.039; rg =0.182). Based on the results of this study, the BWFE could be introduced as a suitable indirect selection criteria for improving egg production because of the higher heritability and a positive genetic correlation with egg production.

Keywords: Genetic parameters, Heat shock protein 70 gene, Homozygote, Taiwan native chicken

Effects of fertilizer, irrigation level and spider presence on abundance of herbivore and carnivore in rice cultivation in Yogyakarta

My Syahrawati1*, Edhi Martono2, Nugroho Susetya Putra2, Benito Heru Purwanto3

1Lecturer in Plant Protection Study Program, Faculty of Agriculture, University of Andalas, Padang, Indonesia

2Lecturer in Department of Plant Pests and Diseases, Faculty of Agriculture, University of Gadjah Mada, Yogyakarta, Indonesia

3Lecturer in Soil Department, Faculty of Agriculture, University of Gadjah Mada, Yogyakarta, Indonesia

 

Abstract

Cultivation techniques that are usually followed in rice cultivation is believed to greatly affect the interaction between arthropods in form of bottom-up and top-down ways, which in turn, affects their diversity and abundance. Beside of that, the spider is generalist predator that has a great potential as a biological pest control agent. This research aimed to determine the effects of spider presence, fertilizer and irrigation level on abundance of herbivore and carnivore in rice cultivation in Yogyakarta, Indonesia (Wates – Kulon Progo). It was done in factorial design using three factors: fertilizers (organic, inorganic, and without fertilizer), irrigation levels (less water = 2 cm, conventional >10 cm), and spider presence (with and without spider). Each combination treatment had three replications.  The results showed that the interaction between fertilizer and irrigation level affected soil PH and total N of rice plant but not to total N of soil. Organic fertilizer with less irrigation decreased the abundance of carnivore but it did not affect the abundance of herbivore. Meanwhile, interaction between spider presence and irrigation level affected carnivore abundance, but not to herbivore generally. The spider presence decreased Delphacidae abundance but did not affect the abundance of Alydidae dan Staphylinidae.

Keywords: Ecological effect, Natural enemies, Parasitoid, Predator, System of rice intensification

The effect of different levels of amino acid and zinc on the quality and quantity of Berseem (Trifolium alexandrinum)

Muhammad Zakirullah1*, Sumayya Innayat1, Tariq Jan1, Muhammad Arif2, Muhammad Ali1, Mehboob Alam3

1Agricultural Research Institute Tarnab, Peshawar, Khyber Pakhtunkhwa, Pakistan

2Directorate of Outreach, Agricultural Research, Khyber Pakhtunkhwa, Pakistan

3Department of Horticulture, The University of Agriculture, Peshawar, Khyber Pakhtunkhwa, Pakistan

 

Abstract

The unavailability of green fodder throughout the year and low quality fodder are some of the main constraints that contribute in low yield of livestock. To overcome the restraint in quality and quantity, an experiment was designed at Agriculture Research Institute, Tarnab – Peshawar to investigate the effect of different levels of amino acid (aspartic acid) and zinc (zinc sulphate) on the quality and quantity of berseem. The experiment was laid out in randomized complete block design with split plot arrangement having three replications. Different levels of amino acid were applied @1000, 2000 and 3000 ml ha-1 to main plot while Zinc was applied @14 and 28 kg ha-1 to sub plot.  Maximum stem height (73.77 cm), branches per stem (20.39), highest percentage of crude protein (17.67 %), crude fiber (33.59 %), dry mater (21.75 %) and green fodder yield (27.84 t ha-1) was recorded in the plots that received the amino acid @ 3000 mlha-1, while the plots that received amino acid at lowest rate i.e. 1000 ml ha-1 yielded the lowest stem height (60.22 cm), minimum number of branches per stem (12.72), low percentage of crude protein (15.85 %), crude fiber (31.21 %), dry matter (19.38 %) and lowest green fodder yield (23.72 t ha-1). Similarly, zinc applied at higher rate of 28 kg ha-1 boosted the stem height (70.89 cm), number of branches (16.62), crude protein (17.86 %), crude fiber (35.01 %), dry matter (21.53 %) and green fodder yield (28.91 t ha-1) compared to zinc applied @ 14 kg ha-1. It is therefore, recommended that while growing berseem amino acid @ 3000 ml ha-1 and Zinc @ 28 kg ha-1 should be applied in order to get good yield and a quality crop.

Keywords: Amino acid, Zinc, Berseem, Quality and quantity

Fatty acids composition profile evaluation of Palm oil in crude oil polluted environment

Emmanuel Ejiofor1*, Ebhohon Shirley2, Adanma Obike2, Bliss Onyedikachi2, Atasie Okechukwu2,  Ajah Obinna2, Kanu Michael2, Ndukaku Omeh2

1Department of Biochemistry, Faculty of Science, Clifford University, Owerrinta, Abia State, Nigeria

2Department of Biochemistry, College of Natural Sciences, Michael Okpara University of Agriculture, Umudike, Umuahia, Abia State, Nigeria

 

Abstract

In this study, the fatty acid profile of oils obtained from palm tree grown on polluted soil in Southern Nigeria. Oil extraction was performed using traditional method. Carotenoid content of palm kernel and palm oil in the test oil showed a significant (p<0.05) decrease when compared to the controls. Mores so, acid and peroxide value increased (p<0.05) significantly in the test oil when compared to the control. The percentage composition of various fatty acids of palm kernel oil from polluted soil were caprilic 2.99%, capric 52.67%, lauric 16.69%, palmitic 8.06% and stearic 19.57%. Fatty acid percentage composition of palm kernel oil from unpolluted soil were caprilic 2.66%, capric 49.18%, lauric 15.17%, palmitic 8.47%, and oleic acid 0.92%. Fatty acid percentage compositions of palm oil from polluted soil were myristic acid 0.28%, palmitoleic 62.09%, oleic acid 37.62%. Unpolluted soil showed lauric acid 0.36%, palmitic acid 44.57% and oleic 55.05%. The absence of palmitic acid and the reduction of oleic acid could be attributed to the impact of the crude oil spillage on the plant. This study shows that crude oil spillage affects composition of fatty acid and oil chemistry, suggesting the use of oil as indicator for environmental pollution.

Keywords: Crude oil spillage, Fatty acid, Palm kernel oil, Palm oil, Pollution, Southern Nigeria

Effects of various doses of copper sulphate on peroxidase activity in the liver, gills, kidney and brain of Cirrhina mrigala

Muhammad Awais Bashir1, Muhammad Javed1, Fariha Latif1*, Faiza Ambreen2

1Department of Zoology, Wildlife & Fisheries, University of Agriculture, Faisalabad, Pakistan

2Department of Zoology, Government College Women University, Faisalabad, Pakistan

 

Abstract

Metal ion pollution of water generates addition of metals in the fish organs that leads towards redox reactions, making free radicals, particularly reactive oxygen species (ROS). All organisms have a strong antioxidant defense system to defend their tissues from injurious effects of ROS produced by metallic ions toxicity. During this study, effects of various doses of copper sulphate (CuSO4) on peroxidase activity in the liver, gills, kidney and brain of Cirrhina mrigala were measured. One year old C. mrigala were subjected to 96-hr LC50, 2/3rd, 1/4th and 1/5th of LC50 of CuSO4, discretely, for period of the 30 days in glass aquariums with three replications for each treatment. The control group of the fish did not receive any metal stress. After 30-day exposure of CuSO4, the fish from all treated groups were sacrificed and their liver, gills, kidney and brain is separated for peroxidase enzyme assay. Peroxidase enzyme activity in the CuSO4 treated fish were compared amongst several treatments and with the control fish. Activity of peroxidase enzyme in all organs of the fish increased significantly (p<0.05) after exposure of CuSO4 as compared to the control fish. Peroxidase activities in the liver, gills, kidney and brain tissues of metal stressed fish were measured as 0.891±0.002, 0.824±0.004, 0.767±0.004 and 0.334±0.004UmL-1, respectively. 

Keywords: Peroxidase activity, Copper sulphate, C. mrigala

Splitting of nitrogen application through growth stages in various sunflower cultivars to improve their vegetative growth and seed yield

Hanaa Khudhaier Mohammed Ali Al-haidary

Department of Field Crops, College of Agricultural Engineering Sciences, University of Baghdad, Karrada District,

Al-Jadiriya, Baghdad, Iraq

 

Abstract

A field experiment was carried out in spring seasons of 2012 and 2013 to increase the efficiency of nitrogen application in spite of that no splitting nitrogen has practiced for the studied cultivars. Five splitting treatments (T1, T2, T3, T4, and T5) of nitrogen application were studied in three sunflower cultivars (Shumos, Akmar, and Zehrat Al-Iraq). The results showed that Shumos cultivar, T4 treatment of nitrogen application and their interaction gave the highest seed yield (6.3 and 6.43), (5.78 and 6) and (6.62 and 6.9 ton ha-1), respectively, on both seasons. The highest significant correlation value belonged to the correlation between seed yield and leaf area (0.85 and 0.87) for both seasons. It can be concluded that splitting of nitrogen fertilizer during growth stages is effective to increase the efficiency of nitrogen application and lead to increase seed yield. Also, leaf area could be a selection criterion to improve seed yield.

Keywords: Helianthus annuus L., Nitrogen, Sunflower, Vegetative growth, Seed yield