Archive
 
Volume 8, Issue 4      October - December, 2020
https://doi.org/10.35495/ajab.2019.06.273

Xylanase production by Aspergillus fumigatus UR01 by solid state fermentation of rice straw
 

Suwattana Singhajutha, Eakaphun Bangyeekhun, Urarux Romruen*

Department of Microbiology, Faculty of Science, Silpakorn University, Nakhon Pathom, 73000 Thailand

Abstract

Sugar generation is important for many industries, such as livestock feed and bioethanol production. Rice straw is an agricultural waste that serves as a good source of sugar. In this study, a xylanase-producing fungus was isolated form compost pile and identified as Aspergillus fumigatus based on the internal transcribing spacer sequence. Xylanase produced by this fungus was characterised. The optimal conditions for fungal xylanase activity were 55°C and pH 5.0. Enzyme activity declined to 45.26% following incubation at 50°C for 2 days. Rice straw was more suitable for xylanase production than other substrates. The filtrate of A. fumigatus grown on rice straw as a substrate was added to rice straw, and reducing sugar production was determined. The maximum reducing sugar production was achieved with incubation at 30°C for 18 h. Xylanase produced by A. fumigatus can be used for rice straw pre-treatment to improve the nutrient content of livestock feed and increase bioethanol production.

Keywords: Xylanase, Rice straw, Agricultural waste, Sugar

Download PDF

FacebookTwitterEmailWhatsApp
 
     
 
 
 
Asian Journal of Agriculture and Biology © 2017  
Asian Journal of Agriculture and Biology is licensed under a 
Creative Commons Attribution 3.0 License.