Asian J Agric & Biol. 2019;7(3):467-473.

The addition of fish Salmon Omega-3 in tris egg yolk diluents on the quality of Simmental bull frozen semen

Wilmientje Marlene Mesang Nalley1*, Tri Sukma Adhe Meidina2, Asep Kurnia3, Raden Iis Arifiantini2

1Department of Animal Production and Technology, Faculty of Animal Science, Nusacendana University. Jl. Adisucipto, Penfui, Kupang, NTT 85001, Indonesia

2Department of Veterinary Cinic, Reproduction and Pathology, Faculty of Veterinary Medicine, Bogor Agricultural University. Jl Agatis Raya Kampus IPB Dramaga, Bogor 16680, Indonesia

3Lembang Artificial Insemination Centre, Lembang, Bandung, Jl. Kayu ambon, Lembang, Bandung, Jawa Barat 40391, Indonesia

Abstract

The sperm plasma membrane is drastically altered by cryopreservation. Therefore, it is necessary to protect the sperm plasma membrane during the cryopreservation process. Omega-3 is a supplement that can protect plasma membrane. This study aimed to compare the quality of Simmental bull frozen semen in Tris Egg Yolk (TEY) diluents with and without omega-3. Semen samples from five Simmental bulls belonged to Lembang Artificial Insemination Center (AIC) were collected using artificial vaginas. The semen samples were evaluated immediatelly (macro and microscopically) after collection. The semen samples then divided into two tubes and diluted with TEY or  TEY with Omega-3 (TEYO) diluents respectively. The semen were packed into 0.25 mL straws and equilibrated at 5 ℃ for 4 hours and froze using automatic freezing machine and stored in liquid nitrogen tank (-196 oC) for further evaluation. The quality of  frozen semen were evaluated after 24 hours of storage. The data were analyzed using independent sample T-test. The results showed that the sperm post-thawing motility in TEY and TEYO diluents were 45.17±1.98% and 47.48±3.55%, respectivelly. No significant difference between TEY and TEYO (P>0.05) was found in the sperm motility, individual score, sperm viability as well as membrane integrity. The research conclude that omega-3 supplementation in Tris egg yolk did not improve the semen quality of Simmental bull after freezing.

Keywords: Frozen semen, Tris egg yolk, Omega-3, Simmental bull, Sperm