Tag Archives: 3 (2)

MODULATION OF IMMUNE SYSTEM BY TAKING PROBIOTIC BACTERIA: ESPECIALLY FOCUS ON LACTIC ACID BACTERIA

Muhammad Shahid Riaz*1, Nagina Batool1, Semhab Saleem1, Abdul Haris2, Tayyaba Shaheen1
1Department of Bioinformatics and Biotechnology, GC University, Faisalabad-38000, Pakistan
2National Probiotic Laboratory, Health Biotechnology Division, National Institute of
Biotechnology and Genetic Engineering, Faisalabad, Pakistan
                                       
ABSTRACT
 
The lactic acid bacteria are gaining more attention as probiotics, because they are generally recognized as safe for consumption. The lactic acid bacteria are commercially very important group of bacteria and have a lot of positive impact on the health of host. The literature studies showed that the lactic acid bacteria exert positive impact on the health to modulate the immune response and protect the host against enteric pathogenic microbes. The lactic acid bacteria enhanced the secretion of IgA and improved the activity of macrophages and T cells. These, also, enhanced the production of interferon’s expression. In short, the consumption of lactic acid bacteria in food modulates the immune system by the secretion of IgA, prevents enteric infectious microbes, and improves the activity of interferon and interleukins.
Keywords: – Immune system, lactic acid bacteria, probiotic, mucosal immunity 

INFLUENCE OF SOIL APPLIED MORINGA LEAF EXTRACT ON VEGETATIVE GROWTH OF CYPERUS ROTUNDUS

Anser Ali1, Nasir Abbas1, M.M. Maqbool1, Tanveerul Haq1,
Muhammad Ahmad*2, and Rashid Mahmood3
1 Faculty of Agriculture, Ghazi University, Dera Ghazi Khan, Pakistan
2Agricultural Training Institute, Karor, Layyah, Pakistan
3Institute of Agricultural Sciences, Punjab University, Lahore, Pakistan
 
ABSTRACT
 
Allelopathy refers to the beneficial or harmful effects of one plant on another plant through the release of secondary metabolites from plant parts in both natural and agricultural systems. Moringa leaf extract (MLE) is generally considered to have enhancing or suppressing effect on the plant growth in a dose dependent manner. Therefore, the current study was planned to evaluate the effects of MLE on the growth of purple nutsedge (Cyperus rotundus). MLE was added into pots three days after transplanting with 25, 50, 75 and 100% concentrations (v/v) whereas distilled water was used as control. Application of MLE at 100% concentration notably improved the root and shoots lengths, shoot fresh and dry weights. MLE application had non-significant effect on root fresh and dry weights, number of nodes and node weights of purple nutsedge. It was concluded that MLE improved the growth of purple nutsedge even higher concentrations rather to suppress it.
Keywords: Moringa oleifera, leaf extract, purple nutsedge, root shoot weight

ASSESSMENT OF YIELD LOSSESS CAUSED BY BACTERIAL BLIGHT OF RICE IN UPPER DIR, KHYBER PAKHTUNKHWA PROVINCE

Mehran Khan1, Abdur Rafi1, Aqleem Abbas*1, Tauheed Ali1 and Akhtar Hassan2
1 Department of Plant Pathology, The University of Agriculture, Peshawar Pakistan
2Department of Agricultural Chemistry, The University of Agriculture, Peshawar Pakistan
 
ABSTRACT
 
The present study was conducted to assess the yield losses caused by bacterial brlight of Rice (Xanthomonas oryzae pv oryza) in Upper Dir a district of Khyber Pakhtunkhwa province of Pakistan during cropping season 2013-2014. Four rice growing locations of district namely Barawal Bandi, Satara, Maidan khwar and Thikar Kot were selected. In Upper Dir disease incidence (%) ranged between 57.14%-75% while disease severity (%) was between 36.04 to 66.12%. Highest disease incidence (75%) and disease severity (66.12%) was recorded from Barawal Bandi location whereas lowest disease incidence and disease severity was recorded from Thikar kot. Similarly maximum 1000 grain weight losses was also recorded in  Barawal Bandi while minimum 1000 grain weight loss was recorded in Thikar kot. It can be concluded that  Bacterial blight of rice was more severe in Barawal location and less severe in Thikar kot.
Keywords: Rice, bacterial blight, % Incidence, % Severity 

OBSERVATIONS ON THE LIFE CYCLE OF HETERODERACRUCIFERAE ON ITS MAIN HOSTS UNDER THREE DIFFERENT CONDITIONS IN IRAN

Habibeh Jabbari* and Gholamreza Niknam
Nematology Laboratory, Department of Plant Protection, University of Tabriz, Tabriz-Iran
 
ABSTRACT
 
The cabbage cyst nematode, Heterodera cruciferae has been reported as one of the pests infecting kohlrabi (Brassica oleracea L. var. gongylodes) and white cabbage (Brassica oleracea L. var. captita alba) in Tabriz vegetable farmlands. In this project, its life cycle on these two hosts in the region was studied. Two cultivars of cabbage commonly grown in the region were cultivated under three different conditions: in naturally infested field, in greenhouse, and in micro plots. During the growing season, the nematode life cycle on the crops was monitored. Samples were collected periodically from host plant roots and soil. The nematode completed two generations and initiated the third on the cabbage. These findings can be used to determine the critical harvesting date of the different cabbage crops to avoid nematode reproduction.
Keywords: Hererodera cruciferae, life cycle, greenhouse, microplot, naturally infected fields.

MORPHOLOGICAL CHARACTERS IN BT COTTON AS INFLUENCED BY THE SPLIT APPLICATION OF NITROGEN APPLIED AT DIFFERENT GROWTH STAGES UNDER THE AGRO-CLIMATIC CONDITION OF DERA GHAZI KHAN, PAKISTAN

Shuaib Kaleem*1, M. Shabbir1 and M. N. Abbas2
1Agriculture Adaptive Research Complex, Dera Ghazi Khan, Pakistan
2Department of Agronomy, College of Agriculture, Dera Ghazi Khan, Pakistan
 
ABSTRACT
 
Nitrogen plays a significant role to improve the growth and yield of cotton. Plant growth, assimilation, morpho-physiological expressions and photosynthate translocation are closely related to nutrients availability. A field trial to evaluate the response of different nitrogen splits on growth and yield components of cotton was conducted under the agro climatic conditions of Dera Ghazi Khan, Pakistan during 2012. Fuzzy seed (@ 20 kg ha-1 of cotton genotype (BT-MNH 886) was sown under Randomized Complete Block Design (RCBD) with three replications. Fixed nitrogen (N) dose (375 kg ha-1) which (375 kg ha-1), was applied in four treatments as a source of nitrogen; T1=5 N splits (@75.00 kg ha-1 per split), T2=6 N splits (@62.50 kg ha-1 per split), T3=7 N splits (@53.57.00 kg ha-1 per split), and T4=8 N splits (@46.87 kg ha-1 per split). The data were recorded on the basis of yield and yield attributes of cotton crop that included plant height, leaf area index (LAI), number of bolls per plant, bolls weight and seed cotton yield. On increasing N splits, vegetative characteristic of cotton i.e., plant height was improved while, features like leaf area index, bolls weight and seed cotton yield increased up to certain level of N splits, T3 (7 splits). Further, N split (8 splits in T4) caused decrease in all these features except for plant height. So the growth and yield performance of cotton genotype (BT-886) was significantly influenced up to 7 splits of nitrogen fertilizer, improving morphological characteristics of cotton crop ultimately enhancing the seed cotton yield.
Keywords:Cotton genotype, nitrogen splits, growth and yield components

CHALLENGES TO BEEKEEPING IN PAKISTAN DUE TO CHANGING PATTERN OF RAINS

Muhammad Asif Aziz
Assistant Professor Department of Entomology,
PMAS-Arid Agriculture University, Rawalpindi, Pakistan
 
Honey and beekeeping have a long history in Pakistan. Bee hives neither demand additional land space nor do compete with agriculture or animal husbandry for any input. The beekeepers need only to spare a few hours in a week to look after their bee colonies. Therefore, there is a great scope for expansion of bee keeping industry in the country. Pakistan currently has around 16000 beekeepers with 0.5 million honeybee colonies. Beekeeping does not compete with other enterprises for resources as the bees use nectar and pollen grains of plants. Therefore, this enterprise can be taken up both at the household and commercial levels to generate substantially more profits.
Floral sources are abundant for beekeeping in most of the areas in Pakistan. Northern Punjab and KPK province of Pakistan are very suitable for beekeeping on account of different ecological zones containing rich bee flora and ideal climatic conditions.  But beekeepers in these areas are facing severe stress to carry on beekeeping activities these days due to continuous non honey production seasons. Factors involved in low honey production are continued raining most of the times, low temperature, delayed flowering and reduced foraging activities of bees.
Last year during October, the beekeepers were able to harvest only 20% of the berry honey, due to sudden rain and wind storms during the middle of the honey flow period, which even took away the bee colonies. As a result flowers containing nectar were dropped and the bees were unable to store honey.
Beekeepers passed the winter season with hope for better conditions during next year but weather conditions also remained unfavorable during current year and frequent rains affected the bee flora drastically. Beekeepers were not able to start queen rearing process for replacing the old queen with young, energetic and healthy queens. New queens are very crucial to maintain the number of colonies and bee population having good inherited characters for the next seasons. Drones necessary for successful queen rearing were killed due to shortage of food in March. Bee colonies started to increase their population one month later than the normal schedule. At the time when foragers were needed to bring and store nectar from the crops, the bees were engaged to consume more food in the form of honey to nourish the young larvae. Abrupt rainfall pattern severely affected the honey to be harvested from Brassica, Citrus, Robinia, Phalai and Carissa plants. A few farmers were able to get honey from Clover and Eucalyptus. Robinia honey in Northern areas remained 25% of the previous crop due to excessive rains. Now the honey flow season is going to end during this month and beekeepers are facing sever trouble to pass the hot summer conditions with weak boxes, without nectar flow bearing heavy input expenses.
Most of our beekeepers are poor people, they get money from middle man to feed and shift colonies at different times of the year and when they produce honey, the borrowed amount is finally adjusted against it. Due to continuous failure in honey production from last October, they are not in good position to bear the expenses of shifting, feeding and medication of bees for the coming season.
Beekeepers are disappointed, due to which many of them are deciding to quit the business and sell the colonies at low rates. Now new beekeepers will emerge with scarce knowledge that will result in low productivity in the coming season. New beekeepers with lack of interest in beekeeping may also lose their colonies due to negligence in hive management Moreover chances of sudden change in weather conditions still exist, and more difficult to wait for next season and get profitable returns in form of abundant honey.
At the same time our crop will be deprived from the pollination services of honey bees, which are provided, free of cost to our farmers and crop yield is increased. Honey bees give 15-20 time more value in the form of pollination then all hive products put together. In USA almond farmers pay 150 dollars per hive per season as a rent for crop pollination to the beekeepers. But our beekeepers provide this service free of cost to our crops. Honey bees are the most efficient pollinators among insect and one bee visits 50-100 flowers in one trip and about 2 million flowers to make one pound honey. Therefore decrease in beekeeping activities is more likely to affect the production in agriculture commodities particularly fruits, vegetables and oilseed crops.
Awareness regarding honeybee health and promoting bee flora are being seriously focused now a days in modern world including USA. In USA, a national strategy to promote the health of honey bees and other pollinators for next ten years has been devised by pollinators health task force formulated under the presidential memorandum of President Obama on May 19, 2015 to promote beekeeping. Similar is the case with other developed countries.

Keeping in view the current scenario the government of Pakistan is requested to provide immediate help to the beekeeping industry to save the beekeepers from monetary losses due to severe climatic change. Moreover the farmers should be advised to cultivate more flowering crops, and government forest departments and other agencies are requested to include trees like Acacia, Robinia, improved varieties of Eucalyptus and Russian olive in their plantation plan along the roadsides, parks and government lands so that food shortage for bees may be addressed to some extent.