Taha A. Kumosani1,2,3, Elie K. Barbour2, Soonham S. Yaghmoor2, Said S. Moselhy4*
1Biochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia
2Experimental Biochemistry Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia
3Production of Bio-products for Industrial Application Research Group, King Abdulaziz University Jeddah, Saudi Arabia
4Biochemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt
*Corresponding author’s email: moselhy6@hotmail.com
Received: 22 November 2025 / Revised: 11 February 2026 / Accepted: 19 February 2026 / Published Online: 02 March 2026
Abstract
The undesired complications and side effects of drugs used in obesity management is the main challenge worldwide. For that, complementary or alternative therapy depending on natural source for obesity management is the target of nutritionist for its safety. The rational of this study to investigate the role of sulforaphane (SR) in amelioration biochemical markers related to oxidative stress, inflammation and obesity hormones high fructose and or high fat diet fed rats. Eight groups of male rats (6 rats/group). Group 1: Rats fed normal diet. GP II: normal rats given SR (10 mg/kg bw). Animals in groups (III-V) were fed either high fructose (HF) or high fat (HFA) or (HF/HFA) diet. However, rats in Groups (VI-VIII): are similar to previous groups but treated orally 10 mg/kg bw SR. Data obtained showed that, SR protected from hepatic abnormalities by normalizing ALT and AST levels. A significant improvement in insulin sensitivity, restoring changes in lipid profile (TC, TG, LDL-c and HDL-c), anti-inflammatory by reducing levels of IL-6 and TNF-α, antioxidant and keeps obesity hormones from alterations compared with untreated rats. Conclusion: supplementation of SR ameliorated biochemical abnormalities induced by HF and or HFA and related hormonal, oxidative stress and inflammations. For that, it is promising to be used in the treatment of obesity-related metabolic syndrome.
Keywords: Sulforaphane, Obesity, High fructose/ high fat, Ghrelin, Leptin, Adiponectin, Rats
