Screening and molecular identification of gelatinase-producing bacteria isolated from Indonesian mangrove ecosystem
A A Prihanto1,2,3*, H Nursyam1,3
1Department of Fishery Product Technology, Faculty of Fisheries and Marine Science, Brawijaya University, Jl. Veteran, Malang 65145, East Java, Indonesia
2 Halal Thoyib Research Center, Brawijaya University, Jl. MT Haryono, Malang 65145, East Java, Indonesia
3Bio-seafood Research Unit, Faculty of Fisheries and Marine Science, Brawijaya University, Jl. Veteran, Malang 65145, East Java, Indonesia
Abstract
The objective of this research was to isolate and identify gelatinase-producing bacteria from several mangrove ecosystems in Indonesia. Soil samples were collected from seven coastal locations of mangrove ecosystem in 2017. The gelatinase-producing bacteria were screened by using screening and confirmation methods. The gelatinase-producing bacteria were further analyzed by using the 16s rDNA molecular method. Ten isolates were diagnosed to be gelatinase-producing bacteria, which belonged to three genera: Lysinibacilus sp, Enterobacter sp. and Proteus sp. The isolated bacteria can further be investigated for the possibility of gelatinase production.
