Asian J Agric & Biol. 2019;7(3):412-422.

Sauropus species containing eudesmin and their DNA profile

Nuttapong Sawasdee1, Arunrat Chaveerach1, Tawatchai Tanee1,2, Kittiya Silawong Suwannakud1,

Pornpimol Ponkham1,3, Runglawan Sudmoon1,4

1Genetics and Environmental Toxicology Research Group, Khon Kaen University, Thailand

2Faculty of Environment and Resource Studies, Mahasarakham University, Thailand

3Faculty of Science and Technology, Mahasarakham Rajabhat University, Thailand

4Faculty of Law, Khon Kaen University, Thailand

Abstract

Some Sauropus and Breynia species were investigated for phytochemicals as well as their DNA profile for genetic relationships. S. bicolor and S. thorelii are important species that contain eudesmin concentrations of 36.445 mg and 32.190 mg from 2 g fresh leaves and 1.5 g dried leaves, respectively, and 10.620 mg from 2 g dried leaves was revealed via high-performance liquid chromatography (HPLC) analysis. The genetic relationships calculated via inter simple sequence repeat (ISSR) dendrogram construction, as shown by similarity indices, revealed Breynia and Sauropus separation accuracies, with S values of 0.71-0.81 between genera, S values of 0.76-0.85 at the interspecific level of Breynia and S values of 0.69-0.80 in the Sauropus species, in agreement with the criteria for category distinction. Barcodes with rpoB and trnH-psbA spacer regions can be used to identify some species of the two studied genera.

Keywords: Barcode, ISSR fingerprint, Sauropus species, Eudesmin, Breynia species