Physiological Studies on Colletotrichum Gloeosporiodes Associated With Wither Tip Disease of Citrus and Its Chemical Control
Salman Ghuffar*1, Muhammad Zeshan Ahmed1, Muhammad Farooq Aslam2,Luqman Amrao1, Sajjad Hyder2
1Department of Plant Pathology, University of Agriculture Faisalabad, Pakistan
2Department of Plant Pathology, PMAS-Arid Agriculture University Rawalpindi, Pakistan
Abstract
Citrus is the second largest fruit produced in the world and Pakistan is among the 12 large producers of the citrus fruit. It is grown in tropical and subtropical climate all over the world. Besides its high economical & nutritional values citrus is attacked by different pathogen such as fungi, bacteria, viruses and nematodes. Among all the pathogens Colletotrichum gloeosporiodes causing citrus wither tip disease is one of the major constrain in citrus production. Therefore current study was conducted to investigate the different physiological characters on the mycelial growth of C. gloeosporiodes and its chemical control. Among different fungal nutrient media Potato Dextrose Agar (PDA) gave maximum mycelial growth (7.9 cm) followed by Citrus leaf extract Agar (CLEA) (4.7 cm) and corn meal Agar (CMA) (3.3 cm).Temperature of 30°C favored maximum colony growth (8 cm) followed by 25°C (7.4 cm), 35°C (4.2 cm), 20°C (3.5 cm) and 15°C (2.3cm). A pH level of 6 favored maximum colony growth (7.9 cm) followed by 5 (7.5 cm), 4.5 (5.3cm), 4 (4.8 cm), 7(3.3cm) and 3(2.4 cm).Among the application of different fungicides such as Topsin-M, Copper oxychloride and Aliette at different concentration (300, 600 and 900 ppm). Topsin-M gave maximum result to inhibit the Maximum mycelial growth inhibition of C. gloeosporioides (1.1 cm) was produced by Topsin-M 9 days after incubation at 30 °C as compared to control (7.6 cm).
Keywords: Physiological studies, Colletotrichum gloeosporioides, chemical control
