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2021(1)      January - March, 2021
https://doi.org/10.35495/ajab.2020.06.339

Nutritional and physical requirements for mycelial growth and basidiocarp production of Trametes elegans from the Philippines
 

Rich Milton R. Dulay1,2*, Abegail A. Alcazar1, Sofronio P. Kalaw1, Renato G. Reyes1,

Esperanza C. Cabrera2

1Center for Tropical Mushroom Research and Development, Department of Biological Sciences, College of Science, Central Luzon State University, Science City of Munoz, Nueva Ecija, 3120 Philippines

2Department of Biology, College of Science, De La Salle University, Taft Avenue, Manila, Philippines

Abstract

This paper highlights the rescue and optimal cultural conditions of the naturally-occurring Trametes elegans. This mushroom has white fan-like leathery fruiting body that commonly grows singly on deadwood. The secondary mycelial culture has off-white, velvety texture, and aerial hyphae. Rice bran broth agar with pH ranging from 5.5 to 6.5, incubated in either lighted or dark, at 30°C, and sealed conditions were the optimum nutritional and physical factors for growth. Both cracked corn and sorghum seeds registered as the best substrates for grain spawn production. Four parts of sawdust + six parts of rice straw significantly produced the highest yield (12.03 g) and biological efficiency (2.41%). Enriched cultivation study using supplemented substrates is currently under investigation with the aim to increase the production.

Keywords: Trametes spp., Secondary mycelia, Basidiocarp, Philippine wild mushrooms, Optimized culture conditions

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