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Volume 5, Issue 4      October - December, 2017

Isolation and identification of phytoplankton from aquatic ecosystems of Lampung Mangroves Center (LMC) as biological feed
 

Tugiyono1*, Jani Master1, Suharso2

1Department of Biology, 2Department of Chemistry,

Faculty of Mathematic and Natural Sciences, University of Lampung, Indonesia

 Abstract

In this paper we studied the isolation and identification of phytoplankton from aquatic ecosystems of Lampung Mangroves Center (LMC) as biology feed. The analysis of sea water quality parameters in the location of the study consisting of pH, salinity, brightness, water temperature, nitrate, nitrite and phosphate, revealed that all the parameters meet the quality standard, except for nitrates and nitrites. Isolation and identification of Phytoplankton taken from aquatic ecosystem of the LMC using net plankton ± 15 was carried out to obtain pure cultures in Live Feed Laboratory of the Center for Marin Culture Lampung, Indonesia. The Phytoplankton samples were isolated in the bacto agar and were added the fertilizers Conwy and Gillard to spur the growth of green and brown algae. The isolation of media was done by scratch method and cultured in a petri dish with upside down position for seven days, and observed every day for evaluating the algae growth. The results showed that there were 14 genera of phytoplankton identified. Six types of 14 genera of phytoplankton identified have potential to be developed as a biological feed: Thalassiosira, Cyclotella, Chaetoceros, Tetraselmis, Nannochloropsis, and Isochrysis, The isolates of phytoplankton produced can be stored in the refrigerator as pure stock cultures and can last for up to 6 months before being used in a medium culture or laboratory culture scale.

 Keywords: Aquatic Ecosystem, Phytoplankton, Isolation, Biological Feed.

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