In this paper we studied the isolation and identification of phytoplankton from aquatic ecosystems of Lampung Mangroves Center (LMC) as biology feed. The analysis of sea water quality parameters in the location of the study consisting of pH, salinity, brightness, water temperature, nitrate, nitrite and phosphate, revealed that all the parameters meet the quality standard, except for nitrates and nitrites. Isolation and identification of Phytoplankton taken fromaquaticecosystemoftheLMC using net plankton ± 15 was carried out to obtain pure cultures inLiveFeedLaboratoryoftheCenter forMarinCultureLampung, Indonesia. The Phytoplankton samples wereisolated in thebacto agar and were added the fertilizers Conwy and Gillard to spurthe growth ofgreen and brown algae. The isolation of media was done by scratch method and cultured in a petri dish with upside down position for seven days, and observed every day for evaluating the algae growth. The results showed that there were 14 genera of phytoplankton identified. Six types of 14 genera of phytoplankton identified have potential to be developed as a biological feed: Thalassiosira, Cyclotella, Chaetoceros, Tetraselmis, Nannochloropsis, and Isochrysis, The isolates ofphytoplankton produced can be stored in the refrigeratoras pure stock cultures and can last for up to 6 months beforebeingusedin amedium culture or laboratoryculturescale.
