Zhamila Baimirzayeva1, Karlygash Korazbekova2*, Aigul Aitbayeva2 1Department of Biotechnology, M.Auezov South Kazakhstan Research University, Shymkent, Kazakhstan 2Department of Biology, Zhanibekov University, Shymkent, Kazakhstan *Corresponding author’s email: korazbekova.karlygash@okmpu.kz Received: 17 February 2026 / Revised: 19 May 2026 / Accepted: 22 May 2026 / Published Online: 11 June 2026 Abstract Pseudomonas spp. are widely recognized as plant growth-promoting rhizobacteria (PGPR) associated with improved plant performance under greenhouse conditions. This study aimed to isolate and characterize Pseudomonas strains from greenhouse soils of the Turkestan region (Kazakhstan) and to evaluate their biochemical traits and effects on tomato (Solanum lycopersicum) growth under controlled conditions. Four fluorescent Pseudomonas isolates were identified based on morphological characteristics and 16S rRNA gene sequencing as Pseudomonas baetica P1-1, Pseudomonas sp. N1-2 and N2-2, Pseudomonas germanica P4. Seed inoculation assays indicated that the tested strains positively influenced germination and early plant growth parameters, with variation among isolates. The strains also exhibited differences in antioxidant activity, radical scavenging capacity, exopolysaccharide (EPS) production, and indole-related traits. Among them, P. baetica P1-1 showed comparatively higher superoxide radical scavenging activity and EPS production, indicating strong functional potential. Compatibility assays revealed no antagonistic interactions among the strains, suggesting their potential use in combined applications. Overall, the results indicate that the studied native Pseudomonas strains possess plant growth-promoting and stress-related functional traits that may contribute to their application as bioinoculants in greenhouse tomato production. Keywords: Pseudomonas, PGPR, Tomato, Greenhouse soil, Bacterial compatibility, Gene sequencing | 
