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2022 (1)      January - March, 2022
https://doi.org/10.35495/ajab.2020.07.391

Investigation of antioxidant and anti-hemolytic properties of Algerian Bunium incrassatum tubers and their effects as diet on histological and biochemical parameters of normal Wistar rats
 

Farid Berroukeche1,2*, Nawel Attoui3, Fethi Toul4, Mohammed Ziane5,6, Nassima Mokhtari Soulimane1, Hafida Merzouk1

1Laboratory of Physiology, Pathophysiology and Biochemistry of Nutrition, Department of Biology, Faculty of Nature and Life Sciences, Earth and Universe, University of Tlemcen 13000, Algeria

2Faculty of Medicine, University of TAHRI Mohammed, Bechar, Algeria

3Laboratory of Vegetal Resources Valorization and Food Security in Semi-Arid Areas South West of AlgeriaDepartment of Biology, Faculty of Nature and Life Sciences, , University of TAHRI Mohammed Bechar, Algeria

4Laboratory of Chemistry and Environmental Sciences, Faculty of Nature and Life Sciences, University of TAHRI Mohammed, Bechar, Algeria

5University Center of Ain Temouchent, Sidi bel Abbes Road, N101, Ain Temouchent, Algeria

6Laboratory of Microbiology Applied to Agri-food, Biomedical and Environment (LAMAABE), Faculty of SNV / STU, University of Tlemcen, Algeria

Abstract

The present study aimed at determining the effectiveness of Bunium incrassatum acetonic and ethanolic tuber extracts as antioxidant (DPPH assay) and anti-hemolytic agents (hypotonic solution, H2O2 and triton X100 assays) and tubers’ powder as dietary supplement on body weight, on histological (liver, thyroid, testes, and kidney) and biochemical parameters (glycaemia, cholesterol, triglycerides, urea, creatinine, HDL, LDL, AST and ALT). In DPPH assay, acetonic extract (IC50=0.02 mg/ml) exhibited by far, higher scavenging potential, even compared to ethanolic extract (IC50=0.19 mg/ml) and ascorbic acid (IC50=0.25 mg/ml) as reference compound. The same extract showed a very good protective effect against hemolysis induced by H2O2 and hypotonic solutions with 76.43%±3.71 and 77.67%±2.07 mg/mL, respectively and closely followed by quercetin as reference compound. The in vivo results revealed that rats receiving orally 15% of B. incrassatum with diet (BID group) during 15 days of experimentation increased significantly their final body weight (98.88%). Then, a significant rise of glycaemia (+17.92%), HDLc (+25%), AST (+36.78%) and ALT (+56.76%) levels associated to a notable decrease of triglycerides (-29.1 %) and creatinine (-12.74%) were observed compared with control animals. These promising findings support the traditional use of B. incrassatum tubers as functional food for human and farm animals, and suggest that their radical scavenging and anti-hemolytic potentials are may be due to the presence of polyphenolic compounds.

Keywords: Bunium incrassatum, Histology, Diet, Antihemolytic, Antioxidant, Wistar rats

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