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2021 (3)      July - September, 2021
https://doi.org/10.35495/ajab.2020.10.520

In vitro anticancer potential of Sacrocephalus latifolius leaves extract and its fractions on human MCF-7 and NCI-H460 cancer cell lines
 

Ikpefan O. Emmanuel1⁎, Ayinde B. Adesina2, Ikpefan J. Ohonmoimen3, Mudassar Azah4 and Farooq A. Dar5

1 Department of Pharmacognosy and Traditional Medicine, Delta State University, Abraka, Nigeria

2Department of Pharmacognosy, University of Benin, Benin City, Nigeria

3 Department of Science Laboratory Technology, Faculty of Science, Delta State University,  Abraka, Nigeria

 4 Dr. Panjwani Centre for Molecular Medicine and Drug Research International Centre of Chemical and Biological    Sciences, University of Karachi, Karachi- 75270

Abstract

 Sacrocephalus latifolius is one of the recipes used in traditional medicine for the treatment of cancer ailment in South-western Nigeria. This work was aimed at evaluating the extract and fractions of this plant against human breast (MCF-7) and lung (NCI-H460) cancer cell lines as well as their antioxidant activity. While the ant-proliferative assay was done using the SRB-assay for the extract (1-250 µg/mL) and fractions (1-100 µg/mL), the DPPH antioxidant assay was recorded at concentrations between 25-400 µg/mL. Against the MCF-7 cell lines, the extract and ethyl acetate fractions recorded growth-inhibitory activities at all concentrations with GI50 of 73.02 and 63.38 µg/mL. While the chloroform fraction recorded cytotoxicities of -1.14 and -6.19 % at 50 and 100 µg/mL with GI50 and TGI of 43.18 and 89.70 µg/mL, the hexane and aqueous fractions were inactive. Similar pattern of activity was recorded against NCI-H460 cell lines with the chloroform fraction exhibiting a higher sensitivity with GI50 and TGI of 24.12 and 54.50 µg/mL respectively. The antiproliferative activity was further corroborated by the higher antioxidant activity (IC50 of 17.91 µg/mL), total phenolic (86.30 mg GAE/g) and flavonoid contents (72.74 mg QUE/g) of the chloroform fraction. The folkloric use of S. latifolius in the treatment of tumor ailments has been justified by this work.

Keywords: Sacrocephalus latifolius, anti-proliferative, cytotoxic, MCF-7, NCI-H460, DPPH, antioxidant, cancer, cell-lines, fractions, SRB-assay, DPPH

5Hamdard A1- Majeed College of Eastern Medicine, Faculty of Eastern Medicine, Hamdard University, Karachi, Pakistan

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