Archive
 
1.0
2021CiteScore
 
36th percentile
Powered by  Scopus
Volume 1, Issue 4      October-December, 2013

GLYCATION INHIBITION BY NIGELLA SATIVA (LINN)–AN IN VITRO MODEL
 
Hira Zafar1, Fatma Hussain1*, Shoaib Zafar2, Riffat Yasmin1
1Department of Chemistry and Biochemistry, Faculty of Sciences, University of Agriculture, Faisalabad,
Pakistan
2Department of Physiology and Pharmacology, Faculty of Veterinary Sciences, University of Agriculture,
Faisalabad, Pakistan
 
ABSTRACT
In present study, an in vitro glycation model comprising both plasma and glucose designed to assess glycation inhibitory potentials of N. sativa seed (NS) extracts. Plasma with several glucose quantities in the presence of NS were incubated for thirty-five days at 37°C. Non-enzymatic glycation (NEG) was quantified by Thiobarbituric acid technique. NEG levels were reduced by NS. Higher NS concentrations significantly (P < 0.05) reduced the NEG demonstrating dose-dependent inhibitory potential of NS. Plasma NEG levels were decreased up to 13% (P< 0.05). NEG inhibition correlated (r=0.784) with NS concentrations. Present glycation inhibitory prospective of NS demands further studies to determine the precise mechanism of action and effective dose of NS for therapeutic interventions.
Keywords: Glycation inhibition, Hyperglycemia, Human plasma, Nigella sativa
Download PDF

FacebookTwitterEmailWhatsApp
 
     
 
 
 
Asian Journal of Agriculture and Biology © 2017  
Asian Journal of Agriculture and Biology is licensed under a 
Creative Commons Attribution 3.0 License.