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Volume 1, Issue 3      July-September, 2013

Enzyme analysis of endophytic new Streptomyces sp.viji10 isolated from velamen roots of orchid plant Vanda spathulata (L.) spreng
 

G. Senthilmurugan @ Viji1*, S. Sekar1, K. Suresh2

1Department of Botany, Thiagarajar College, Theppakulam, Madurai, Tamilnadu State, India.

2Department of Botany, Saraswathy Narayanan College, Perungudi, Madurai, Tamil Nadu State,

India.

*Corresponding author: e-mail: viji.m6@gmail.com

 

ABSTRACT

The present study deals with enzyme analysis of endophytic new Streptomyces sp.viji10 isolated from the velamen roots of Vanda spathulata is an epiphytic orchid plant. The plant root samples were collected from Alagar kovil Hills, Madurai district, Tamilnadu state, India. An endophytic Streptomyces sp was isolated by using NA (Nutrient Agar) medium; it was then identified by 16S rRNA gene sequencing analysis. The endophytic actinomycete culture to produce different enzymes was analyzed. The endophytic actinomycete culture has the ability to produce cellulase enzyme (except amylase, laccase, lipase and protease). The result of the study suggested that endophytic Streptomyces sp associated with velamen roots of Vanda spathulata plant are potential source of cellulase enzyme production.

Keywords: Enzyme analysis, Streptomyces sp.viji10, Vanda spathulata, Velamen roots

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