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Volume 2, Issue 4      October-December, 2014

EFFICACY OF DIFFERENT CHEMICALS FOR THE IN VITRO CONTROL OF ALTERNARIA SOLANI ISOLATES COLLECTED FROM VARIOUS AREAS OF PESHAWAR
 
Ihsan Ul Khaliq*, Afaq Ahmad and Haneef Raza
Department of Plant Pathology, The University of Agriculture, Peshawar-Pakistan.
 
ABSTRACT
A research was conducted at the department of Plant Pathology Kyber Pakhtunkhwa agricultural university Peshawar to evaluate different fungicides in vitro for the control of Alternaria solani isolated from infected tomato leaves. Three isolates of A.solani were obtained from the department of plant pathology Khyber Pakhtunkhwa agricultural university peshawer. For the In vitro control of A.solani, three fungicides i.e., Dithane M-45, Topsin-M and Cupravit were tested by Poison food technique at concentration of 500mg/L. In case of Drench method, a fungicide suspension was prepared by dissolving 0.05g of each fungicide in sterile distilled water to give a concentration of 500mg/L whereas control treatment had no fungicide. The pure culture of three isolates were cut with 5 mm diameter sterile cork borer. These inoculm discs were treated with fungicides by dipping in the fungicides suspension for 1-2 minutes and were seeded in the centre of each petri dish. The petri dishes were incubated at 250C for 10 days. In case of poison food technique, the lowest colony diameter (32.53mm) was recorded in treatment with Dithane M-45 followed by Cupravit (46.26mm) and Topsin M (55.93mm). Dithane m-45 also inhibited spore concentration significantly (42.45%) where the mean highest colony growth (62.76mm) was in control.
Keywords: Alternaria solani, fungicides, Poison Food Technique, Drench Method
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