Archive
 
Volume 2, Issue 3      July-September, 2014

EFFECT OF PHOSPHATE SOLUBILIZING BACTERIA ALONG WITH ACC-DEAMINASE ON WHEAT GROWTH UNDER AXENIC CONDITIONS
 
Shabana Ehsan1*, Ifra Saleem1 and Hafsa Zafar2
1 Soil Chemistry section, ISCES, Ayub Agricultural Research Institute, Faisalabad
2 Biochemistry Section, PHRC, Agricultural Research Institute, Faisalabad

ABSTRACT
Phosphorus is one of the major plant growth limiting nutrients and in Pakistan its deficiency found in 90% soils due to alkaline calcareous nature of soils. Plant growth promoting rhizobacteria are efficient in supplying nutrients to plants by solubilizing the unavailable/insoluble (P) in soil or by promoting extensive root growth through 1-aminocyclopropane-1-carboxylate (ACC-deaminase) activity. This study was conducted to assess the effects of phosphate-soluibilizing rhizobacteria and their impact on wheat growth under axenic conditions. Initially rhizobacteria (S1 to S10) were isolated through enrichment by rock phosphate [Ca3 (PO4)2] and ACC as sole P and N source, respecitively. After isolation, identification and characterization of these strains was done for root colonization, P-solubilization, in vitro ACC-deminase ctivity, auxin production and chitinase activity. The results revealed that rhizobacterial isolate S8 was best strain and showed 2.6 fold higher fresh weight, 2.7 fold higher shoot length and 4 fold higher root length of wheat over uninoculated control. Similarly, S8 was best root colonizer in wheat rhizosphere (8.0 x 10-7 cfu g-1) while isolate S9 showed the highest P- solubilization i.e. 14.36 ppm, S2 showed maximum ACC-deaminase activity (1.8 μmol α-ketobutyrate) and S4 showed highest auxin production (36.77 mgL-1). This study concluded that S8 strain efficiently solubilize phosphate alongwith ACC-deaminase trait which improved the growth of wheat under axenic conditions.
Keywords: Rock phosphate, Phosphate Solubilizing bacteria (PSB), ACC-deaminase.
Download PDF

 
     
 
 
 
Asian Journal of Agriculture and Biology © 2013  
Asian Journal of Agriculture and Biology is licensed under

.