Category Archives: b_original_articles

Chandrasekar R¹*, Murugan K², Bhatacharyya A³

¹Department of Biochemistry and Molecular Biophysics, 238, Burt Hall, Biotechnology Core

Facility, Kansas State University,   Manhattan 66506, KS, USA

²Department of Zoology, School of Life Sciences, Bharathiar University, Coimbatore-641 046,

Tamilnadu, India
³Vidyasagar  College for Women,  Department of Zoology, Sankar Ghosh Lane, Kolkata-

700006, India.

*Corresponding author: e-mail: biochandrus@yahoo.com

ABSTRACT

Determination and expression of sex in insects are important not only in basic biology but also in practical applications. The external morphological characters were evaluated for the determination of sex in Amsacta albistriga Walk. (Lepidoptera:Arctiidea) during the pupal stage for laboratory studies. Among the various parameters examined, size, length, weight, location of genital pore, anal pore and the ratio of distance between genital and anal pores are found to be reliable criteria for sexing pupae.  In addition, Storage protein1 (SP1) appropriately called as “female specific protein” could contribute towards the confirmation of the individual’s sex.  In A. albistriga, the SP1 was separated on 10% SDS-PAGE and their identity was confirmed using immunoblot analysis. This paper deals with two issues simultaneously: A simple technique to separate male and female pupae of A. albistriga in livable conditions for further behavioral, physiological and molecular studies. The efficient contribution of SP1 as a biochemical marker and its exploitation in reproduction and integrated pest management.

Keywords: anal pore, female specific protein, genital pore, storage protein 1, reproduction

Muhammad Abu Sufyan¹, Azhar Mahmood^1,5,  Anser Ali^2,3, Muhammad Mudassar Maqbool²

and Muhammad Ahmad^4*

¹Department of Agronomy, University of Agriculture, Faisalabad-38040, Pakistan.

²UAF, Sub-Campus, College of Agriculture, Dera Ghazi Khan, Punjab, Pakistan

³Department of Crop Science and Biotechnology, Dankook University, South Korea

⁴Agriculture Adaptive Research Complex, Dera Ghazi Khan, Pakistan

⁵Adaptive Research Farm, Sheikhupura, Pakistan

*Corresponding author: e-mail: mahmada2003@yahoo.co.uk

ABSTRACT

With the objective to investigate the growth and yield response of three wheat varieties at two different sowing dates, a field study was conducted at Adaptive Research Farm, Sheikhupura, Punjab, Pakistan. The experiment was laid out in a randomized complete block design with split plot arrangement and replicated four times. The cultivars were Inqalab-91, Uqaab-2000 and AS-2002, whereas sowing dates were 24.11.03 and S_­2 = 14.12.03, respectively. The sowing date was randomized in main plot and cultivars were randomized in sub plot. The study revealed that increase in all yield components (fertile tillers, number of spikelets per spike, number of grains per spike and 1000-grain weight) of wheat was observed in November sown crop. The cultivar AS-2002 performed better than the rest of two cultivars. Cultivar AS-2002 with the highest grain yield of 3647 kg ha^-1 and early (November) sowing were considered suitable for farmers under agro ecological conditions of Sheikhupura.  

Keywords: Wheat cultivars, Sowing date, Growth, Yield components

Alfredo Socorro García¹*, Francisco García Reina², Yulexis Pino Franco³and Danilo Díaz Páez³

¹Department of Plant Physiology and Post-harvest, Institute of Fundamental Researches on Tropical Agriculture “Alexander Von Humboldt” INIFAT, Santiago de las Vegas, Havana, Cuba

²D epartment of Physics, University of Ciego de Ávila, Carretera de Morón, Ciego de Ávila, Cuba

³Production and Development Center AGROF.  Finca Las Glorias, carretera a Amaro, Santo Domingo Villa Clara, Cuba

  *Corresponding author: e-mail: dpfisiologia@inifat.co.cu

ABSTRACT

The effects of stationary magnetic field on seeds are studied using two soybean varieties and three magnetic induction values: 100, 150 and 200 mT during 30 seconds of exposure. The germination percent, root and stem length as well as fresh and dry mass were measured under laboratory conditions, at the second week after sowing. The plant grown from treated samples (100 and 150  mT) and non-treated (B=0) were also evaluated under field conditions. The values of plant height (65 days after sowing) and the values of pod number, pod length and yield at 110 days (at the end of the plant cycle), were obtained. The results showed the enhanced of the germination percentage and root length of the Conquista and Incasoy-24 varieties treated with stationary magnetic field of 150 mT and 100 mT respectively. The rest of the parameters evaluated did not show differences with respect to the non-treated sample. These respective exposure regimes also provoked a significant stimulation on the yield and an inhibition on the plant height at 110 days, while the magnetic treatment of 100 mT enhanced significantly the plant height of Incasoy-24 variety at 65 days after sowing (P<0.05).

Keywords: Bioelectromagnetics, Legume, Plant, Yield

Tariq Ali, Sajid Mahmood, Muhammad Yahya Khan*, Ana Aslam, Muhammad Baqir Hussain, Hafiz Naeem Asghar and Muhammad Javed Akhtar

Institute of Soil and Environmental Sciences, University of Agriculture, Faisalabad-38040, Pakistan

*Corresponding author: e-mail: yahya.1311@hotmail.com

ABSTRACT

Toxic level of cadmium (Cd) has been built up in soil profile due to over fertilization, industrial activities and irrigation with city wastewater. One of the remediation strategies of such contaminated soil is phytoextraction by using hyper-accumulator plants. Metal stress hinders plant growth in contaminated soils even hyper-accumulator plant cannot attain sufficient biomass for effective phytoremediation. Plant growth promoting bacteria and auxin help plants to cope with metal stress. A pot experiment was conducted where the efficacy of four different Cd tolerant bacterial isolates alone and in combination with auxin (10^-3 M) was evaluated to promote spinach growth in Cd contaminated soil. Results revealed that Cd contamination severely decreased the spinach growth. However, bacterial inoculation and auxin application improved the spinach growth and Cd uptake compared to control. But combined application of bacterial isolates and auxin was more pronounced for enhancing fresh and dry mass, and phytoextraction of Cd.  Bacterial inoculation along with auxin increased the fresh and dry weight up to 261 and 45%, respectively, over control. Moreover, combined use of bacteria and auxin also enhanced the uptake of Cd by spinach up to 5.36 folds compared to control. Thus, synergistic use of bacteria and auxin could be a novel approach for improving plant growth under metals stress as well as for meaningful phytoremediation of metals contaminated soils.

Keywords: Spinach, Phytoextraction, IAA

Kiran Tauseef Bukhari* and Humaira Zafar

Department of Pathology, Al-Nafees Medical College, Isra University Islamabad Campus, Islamabad, Pakistan

*Corresponding author: e-mail: kiran.tauseef@yahoo.com

ABSTRACT

Hepatitis C viral (HCV) infection is amongst the serious health issues in Pakistan. The early case recognition and hence its management via interferon and anti-virals can be helpful to reduce the high morbidity and mortality rates from the said infection in Pakistan. Objective of this study was to assess the variation in different hematological parameters amongst the newly diagnosed Hepatitis C viral (HCV) patients by blood complete picture examination. A Descriptive study was carried out on randomly selected 182 patients at the Pathology Department of Al-Nafees Medical College and Hospital, Islamabad, Pakistan. A simple random sampling technique was adopted for this study. It was an open study and patients of age range between 18 to 40 years were included. An informed consent followed by a questionnaire filling was the important prerequisites of study in order to include or exclude the specific patient. The samples were finally analyzed by immune chromatographic test (ICT) for the detection of anti-HCV antibodies. The blood complete picture examination was carried out to assess the variation in hematological parameters. Out of the total 182 patients, 122 were found to be positive for anti-HCV antibodies presence. The results of current study had shown that 11.98 + 0.003 gm/dl and 123 +0.001 mm³ were the mean values of hemoglobin (Hb) and platelets respectively. While 8.11 + 0.001 mm³ was the mean value for TLC. It was concluded that thrombocytopenia is the most common feature observed in newly diagnosed HCV positive cases.

Keywords: Hepatitis C, Hemoglobin, Platelets, Total Leukocyte Count

Faisal Nadeem¹, Riaz Ahmad¹, Muhammad Ishaq Asif Rehmani², Anser Ali², Muhammad Ahmad ³* and Javaid Iqbal⁴

¹ Department of Agronomy, University of Agriculture, Faisalabad, Pakistan

²Department of Agronomy, College of Agriculture, Dera Ghazi Khan, Pakistan

³Agriculture Adaptive Research Complex, Dera Ghazi Khan, Pakistan

⁴ Adaptive Research Farm, Gujranwala, Pakistan

*Corresponding author: e-mail: mahmada2003@yahoo.co.uk

ABSTRACT

A field experiment was conducted to study the effect of different application timings of P in combination with Zn and P alone on rice at Adaptive Research Farm, Gujranwala, Pakistan during the year 2010. The experimental treatments include:  P application at the time of last puddling,  P application just before transplanting, P application 10 days after transplanting, P application 20 days after transplanting, P + Zn application at the time of last puddling, P + Zn application just before transplanting, P + Zn application 10 days after transplanting and P + Zn application 20 days after transplanting. Different application timings of P in combination with Zn significantly reduced the sterile percentage, when application delayed which resulted in more normal kernels as compared to application of P alone and late one. Maximum abortive and opaque kernels were observed when P application was done just before transplanting. Maximum chalky kernels were perceived when P+Zn were applied 10 days after transplanting.Regarding concentration of P and Zn in rice straw and grain significant difference was observed in all treatments. The highest Zn concentration in grain and straw was observed in P + Zn application at 20 days after transplanting, while minimum Zn concentration was observed in all treatments where P alone was applied in different timings. P concentration in grain and straw was observed near seedling transplanting time more as compared to late applications of P alone and in combination with Zn. It is concluded that Zn is essential nutrient for anaerobic rice. More ever best timing for P application in rice crop is at time of last puddling. Combine application of Zn and P in rice crop at the time of last puddling, resulted in higher yield.

Keywords: Rice, Time of application, Zn, P, Yield and Qualitative Characteristics

Muhammad Zubair^1*, Ijaz Ahmad¹, Maqbool Ahmad¹ and Zahid Iqbal²

¹Department of Theriogenology, University of Agriculture Faisalabad, Pakistan

²Department of Pharmacology, Al-Nafees Medical College, Isra University Islamabad Campus, Islamabad, Pakistan

*Corresponding author: e-mail: drzubairabbasi@gmail.com   

ABSTRACT

The functional integrity of spermatozoa in various domestic animals like horse, cattle and swine is evaluated by hypo osmotic swelling test. The hypo osmotic swelling test has not been tested in the fresh semen of Nili-Ravi buffalo bull.  The aim of the present study was evaluation of membrane integrity by establishing the best hypo osmotic swelling solution. Ten solutions with different osmolarities were used: 70(HS1), 90(HS2), 100(HS3), 120(HS4), 140(HS5), 150(HS6), 190(HS7), 230(HS8), 280(HS9) and 300 (HS10). Twenty samples were collected (ten from each bull) with forty eight hours interval. The collected semen was placed in water bath at 37°C and was evaluated macroscopically and microscopically for color, volume, mass activity and motility. After evaluation 0.1 ml of semen was mixed with 1ml of hypo osmotic swelling solutions and incubated for one hour at 37°C. A total of two hundred sperms were counted in five fields and sperms were classified as swollen and strongly swollen. The percentages of coiled spermatozoa in ten hypo osmotic swelling solutions mentioned above were 18.3%, 23.5%, 37.3%, 41.4%, 50.2%, 57.5%, 38.6%, 26.0%, 13.9% and 6.2% respectively. The percentages of strongly swelling solutions were 9.2%, 12.0%, 21.6%, 32.8%, 31.0%, 39.1%, 29.3%, 21.7%, 10.0% and 5.7% for ten solutions respectively. HS6 was greater to HS1, HS2, HS7, HS8, HS9 and HS10 in increasing the ability of swelling (P<0.05). Similarly HS6 showed greater ability of swelling as compared to HS1, HS2, HS8, HS9 and HS10 (P<0.05).These results indicated that the solution having 150 mOsm/L was the best for functional membrane integrity of spermatozoa in fresh semen of Nili-Ravi buffalo bull semen.

Keywords: Hypo osmotic swelling test, Functional membrane integrity, Spermatozoa, Nili-Ravi bull

Anjali M. Shrestha*

Botany Department, Trichandra College, Tribhuvan University, Kathmandu, Nepal

*Corresponding author: e-mail: rajeshanjali_hanoi@yahoo.com

ABSTRACT

Trifolium repens is an important fodder crop in subtropical and temperate pastures of Nepal where it plays an important role in pasture management.  It was introduced in 1860 in Nepal from England and has since establishment in and o  utside of such pastures. The species has been known for exhibiting phenotypical differences but no attempt has so far been made for identification of its different variations. This is the first attempt where identification of varieties of Trifolium repens collected from different parts of Nepal was carried out.  “Flower color and leaf markings” singly or in combination was found to be the chief indicators for varietal differentiation. Morphological study of each variety of Trifolium repens, collected from Kirtipur, Dhunche and Chandanbari was carried out. Highest and lowest number value and average highest total value of different morphological parameters, were measured. SPSS/PC+, one way analysis of variance at 0.05 levels showed significant variations in nodule size, stipule length, petiole length, petiolule length, leaflet size, head length, floret number, peduncle length, floret length, calyx length, anther size and ovule number among eight varieties growing at Kirtipur, six at Dhunche and six at Chandanbari. The morphological evidence on the basis of above identifying phenotypic characters of Trifolium repens, growing at Krtipur, Dhunche and Chandanbari helps to conclude that eight varieties of Trifolium repens were identified using these criteria (i.e. Intra-specific diversity) in Nepal.

Keywords: Identification, Varieties, Flower color, Leaf markings