Category Archives: b_original_articles

Original Articles

Utilization of mango peel in development of instant drink

Anwaar Ahmed1*, Hafiz Muhammad Rizwan Abid1, Asif Ahmad1, Naeem Khalid2, Sahar Shibli3,

Rai Muhammad Amir1, Arshad Mahmood Malik4, Muhammad Asghar5

1Institute of Food and Nutritional Sciences, PMAS-Arid Agriculture University, Rawalpindi, 46300, Pakistan

2Deputy Secretary (Planning), Department of Agriculture, Government of Punjab, Lahore, Pakistan

3Food Science Research Institute, National Agricultural Research Centre, Islamabad, 44000, Pakistan

4Department of Economics, PMAS-Arid Agriculture University, Rawalpindi, 46300, Pakistan

5Post Harvest Research Center, Ayub Agriculture Research Institute, Faisalabad, Pakistan

Abstract

Worldwide, fruit and vegetable processing industries are generating massive agro-industrial by-products. The industrial processing of mango generates a lot of by-products. Usually the major is with mango peel having a 7-24% portion of total fruit and not normally utilized. In the present research, mango peel was utilized in the formulation of instant drink as an experiment to harvest its nutrients. The mango peel was dried in the dehydrator and ground to obtain the fine quality powder. The mango peel powder (MPP) obtained was evaluated for its nutritional profile. The powder was added in the instant drink 5 g (T1), 10 g (T2) and 15 g (T3) concentration per 250 mL along with water, sugar, citric acid and permitted color and flavor. The instant drink was evaluated at the 0, 30 and 60th-day storage intervals for various physicochemical characteristics. Results showed that mango peel is an abundant source of moisture, ash, fat, protein, carbohydrate, and crude fiber.  The antioxidant activity, vitamin C and total phenolic content were 73%, 14.8 mg/100g, and 81.3 mg/g GAE, respectively. The mean values for total sugars, Total Soluble Solids (Brix), Titratable Acidity etc. differed considerably among all the treatments. During the storage interval from 0 to 60 days a significant decrease in pH, total soluble solids and total sugars, free radical scavenging activity and total phenolic content were observed, while the acidity of the drinks was gradually increased. The drink with 5 g mango peel powder showed the best sensory attributes in terms of flavor, taste and mouthfeel compared to 10 g and 15 g.  This research can be helpful to utilize mango peel waste into food products to harvest the functional, nutraceutical and bioactive compounds. 

Keywords: Food waste, Functional food, Storage, Physicochemical properties

Morphologic characterization of Sarcocystis spp. histologically detected from the diaphragm and tongue of Rattus norvegicus and Rattus tanezumi

Cristina G. Cabanacan-Salibay1,2,*, Florencia G.  Claveria3, Hazel Anne Luyon-Tabo1,2, Steven Paulo C. Salibay4, Augusto C. Sumalde5,  Mario S. Torres1,2,   Julieta Zabal-Dungca6

1Biological Sciences Department, College of Science and Computer Studies, De La Salle University, Dasmariñas, Cavite, Philippines

2Graduate Studies Department, College of Science and Computer Studies, De La Salle University, Dasmariñas, Cavite, Philippines

3Biology Department, College of Science, De La Salle University, Taft Avenue, Manila, Philippines

4Department of Biological Sciences, College of Arts and Sciences, Cavite State University, Main  Campus,  Indang Cavite, Philippines

5Institute of Weed Science, Entomology and Plant Pathology, College of Agriculture, University of The Philippines, Los Baños, College, Laguna, Philippines

6School of Science and Technology, Centro Escolar University, Mendiola, Manila, Philippines

Abstract

This study focused on the detection of Sarcocystis spp. in two species of rats, Rattus norvegicus and R. tanezumi collected from agricultural area in Dasmarinas, Cavite.  This aimed to corroborate the presence of Sarcocystis spp. in different species of rats found in the agricultural area. Further the establishment of different Sarcocystis spp in Rattus spp. based on the parasite’s morphologic characterization is also emphasized. Sixty-nine rats (36 R. norvegicus and 33 R. tanezumi) were collected through trapping. Individual rodent autopsy for host identification was performed by determining the morphological differences and external measurements prior to dissection.  Tissue samples were examined for the presence of white rice-grain sized nodules. Morphological characteristics of the cysts, particularly the size, shape, and presence of protrusions were noted.  Statistical analysis using ANOVA for the significant difference on the number of infected rats per species and across muscle type was done at p≤0.05. Infection in R. tanezumi was higher (48.48%) as compared to R. norvegicus (41.67%), the difference however was not significant. Sarcocysts burden was higher in the diaphragm in both rat species. Generally, higher parasite load was observed in R. norvegicus.   In both rat species, tissue cysts ranged from spindle-shaped/fusiform to globular and oval-shaped. The sarcocysts in the diaphragm were more varied. Consistently observed in the tongue were globular–shaped sarcocysts while either fusiform- and globular-shaped sarcocysts in the diaphragm. These differences suggest infection with more than one species of Sarcocystis. Hence, the presence of Sarcocystis spp. confirmed the infectivity of the parasite to Rattus spp. Moreover, the different morphologic characteristics observed on Sarcocystis prove the possibility of different species of the parasite harboring the Rattus spp.

 Keywords: Agriculture, Diaphragm, Rattus sp, Sarcocystis sp, Tongue

Physiology, yield and quality of soybean as affected by drought stress

Oqba Basal*, András Szabó

Department of Crop Production and Applied Ecology, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, Debrecen, Hungary

Abstract

Drought stress is one of the most hazardous abiotic stresses increasingly affecting drought-sensitive crops like soybean. An experiment was conducted in Debrecen, Hungary in 2018 to investigate the influence of drought stress on physiology, yield and seed quality of three soybean cultivars different in maturity timing. Drought-stressed treatments of the three cultivars showed less normalized difference vegetation index (NDVI) and leaf area index (LAI) compared to fully-irrigated counterparts, whereas relative chlorophyll content (SPAD) did not measurably differ. Drought reduced the yield of the three cultivars, however, yield of middle maturity group cultivar was better than that of early maturity group cultivars, and the same conclusion was obtained from non-stressed treatments. Protein concentration changes were negligible.

Keywords: Leaf area index, Normalized difference vegetation index, Protein concentration, Seed yield, Soybean

Acacia plantation in different quality sites and fertilization in Northeast Vietnam

Tran Van Do*

Silviculture Research Institute, Vietnamese Academy of Forest Sciences, Hanoi, Vietnam

Abstract

The impacts of site quality and fertilization on the growths of acacia plantation have become important in plantation management. It could provide information on which site and how much fertilizer should be applied, contributing to sustainable plantation management. In this study, two sites of different conditions of soil depths (30-40 cm/shallow soil site and 70-80 cm/deep soil site) and of rock contents (< 10% and > 45%); and fertilizing 200 g NPK (16:16:8)/tree/year and control (non-fertilization) were experimented in an Acacia mangium plantation in northeast Vietnam. The plantation was established in September 2017, fertilizer was applied in Junes 2018 and 2019. Site conditions and fertilization significantly affected the growths of 2-year-old plantation. In shallow soil site, the plantation in fertilization treatment achieved 498% basal area and 595% aboveground biomass of plantation in non-fertilization treatment. The difference among control and fertilizing 200 g NPK/tree in deep soil site, and fertilizing 200 g NPK/tree in shallow soil site was not significant. It is concluded that fertilizing NPK to acacia plantation in the present study site is necessary for shallow soil site even trees become older, while in deep soil site fertilization is required only in the first year to support initial growths.

Keywords: Fertilization, Plantation growth, Shallow soil, Site condition, Soil nutrient pool

Bioaccumulation potential of In vitro regenerated plants of Ceratophyllum demersum against Chromium – A lab study

Muhammad Aasim1*, Senar Aydın2, Mehmet Karataş3, Mehmet Emin Aydın4, Canan Soğukpınar4, Canan Sevinc3

1Department of Plant Protection, Faculty of Agricultural Sciences and Technologies, Sivas University of Science and Technology, 58140, Sivas, Turkey

2Department of Environmental Engineering, Faculty of Engineering and Architecture, Necmettin Erbakan University, Konya, Turkey

3Department of Biotechnology, Faculty of Science, Necmettin Erbakan University, Konya, Turkey

4Department of Civil Engineer­ing, Faculty of Engineering and Architecture, Necmettin Erbakan University, Konya, Turkey

Abstract

Phytoremediation of contaminated environment using hyperaccumulator plants is considered as ecofriendly and cost-effective technique. Aquatic plants like Ceratophyllum demersum (Coontail) is used for the phytoremediation of aquatic environment contaminated by heavy metals. In this study, in vitro regenerated plants of C. demersum were exposed to different concentrations of Chromium (Cr) (0, 3, 6, 9, 12 and 15 mg L-1) for different exposure time (1, 3 and 5 days) in order to investigate the phytoremediation potential. The plants contained in Cr containing liquid medium were cultured in growth room having 16 hr light photoperiod aided by white Light Emitting Diodes (LEDs) and temperature of 24±1 0C. The plant samples were taken out from liquid medium after 1, 3 and 5 days for taking data regarding fresh  weight, dry weight, Cr uptake by plants and bioconcentration factor (BCF). The clear relationship between Cr concentration and exposure time on phytoremediation was revealed. Both fresh and dry weight of plants was recorded higher at variable Cr concentration compared to control plants. The exposure time of 3 days to Cr caused the maximum Cr uptake, followed by further uptake of Cr at relatively slow rate after 5 days. The highest Cr uptake and BCF was achieved from medium provided with 12 mg L-1 and 3 mg L-1 of Cr respectively. Comparison of exposure time exposure time × Cr concentration revealed the highest Cr uptake (9145 mg kg-1) and BCF value (2076.5) from the combination of 12 mg L-1 × 5 d and 3 mg L-1 × 5 d respectively. The results revealed that in vitro regenerated plants of C. demersum can be used for phytoremediation of Cr and possibly use against other heavy metals.

Keywords: Bioconcentration factor, Chromium, C. demersum, Phytoremediation, Water

Effectiveness of vermicompost with additives of various botanical pesticides in controlling Plutella xylostella and their effects on the yield of cabbage (Brassica oleracea L.var. Capitata)

Nurhidayati1*, Masyhuri Machfudz2, Abdul Basit1, Rose Novita Sari Handoko1

1Department of Agrotechnology, University of Islam, Malang, East Java, Indonesia

2Department of Agribusiness, University of Islam, Malang, East Java, Indonesia

Abstract

The larva of Plutella xylostella causes damage to cruciferous plants by feeding the tissue between the leaf surfaces. To increase the production of organic cabbage vegetables, it is necessary to develop botanical pesticides. This study aims to describe the effect of vermicompost with additives of various botanical pesticides on the intensity of the moth diamondback attack and cabbage yield. The pot experiment employed randomized block design, with six treatments including plants: (1) without vermicompost and pesticide (control), (2) with synthetic pesticide application (SP), (3) with the application of the mixture of vermicompost and neem (V+N), (4) with the application of the mixture of vermicompost and papaya (V+P), (5) with the application of the mixture of vermicompost and marigolds leaves (V+M), (6) with the application of a mixture of vermicompost and soursop leaves (V+S). Each treatment has five replications and three pot samples per replication. The results showed that at the end observation of attack intensity of P. xylostella, treating cabbage with marigold leaves (20.26%) and soursop leaves (17.50%) had the same effectiveness with synthetic pesticide (21.86%) and significantly different with the other treatments (P < 0.05) by intensity attack of 29.69% (neem leaves) and 24.31% (papaya leaves). Treating cabbage with either soursop leaves or marigold leaves increases the size of the cabbage head, marketable yield, fresh weight and dry weight of biomass total and significantly different from the other treatments (P< 0.05). The results suggest that application of vermicompost with botanical pesticide additives is effective to control P. xylostella in the production of organic vegetables. 

Keywords: Vermicompost, Botanical pesticides, Plutella xylostella, Cabbage

Biodegradation of plastic wastes by confused flour beetle Tribolium confusum Jacquelin du Val larvae

Hind Suhail Abdulhay*

Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq

Abstract

As a consequence of increasing the production of plastics which accumulate substantial quantities of plastic wastes in the natural environment and in landfills that could persist for centuries. The ability of confused flour beetle Tribolium confusum larvae to consume and biodegrade different types of plastics were investigated. The experiment was performed by starving the larvae then exposed to three different types of plastic (polystyrene (PS), polyethylene foam (PE), and ethylene-vinyl acetate (EVA) as diet in comparison to larvae reared on the conventional diet of wheat flour. The larvae were monitored under controlled conditions then the survival rate, the mass losses for both larvae and plastics resulting from the diet on plastic as a function of time (1, 14, 21 and 30 days) were measured. The results showed that with the increase of time; the averages of larvae survival on all types of plastic were decreased compared with control. The highest larval survival rate was found in the PS diet (70%), while the least was with larvae fed on EVA (30%) after 30 days. Decreasing in the mass weight of the larvae was confirmed during the study, which indicates that plastic materials are not an efficient source of energy for larvae except their survival. The mass loss was 26.2, 31.4 and 45.8 % recorded for larvae fed on PS, PE, and EVA respectively. The study recorded that T. confusum has the ability to degradation plastic, which can reduce the pollution caused by different types of plastic wastes.

Keywords: Tribolium confusum, Biodegradation, Plastic waste, FTIR

Role of endomycorrhizae, rhizobacteria and compost to improve phosphorus availability in onion

Faiza Shahzad1*, Hafiz Naeem Asghar1, Zain Mushtaq1, Amina Hadayat1, Naqshe Zuhra1, Rizwan Ahmad2, Muhammad Arif Ali3

1Institute of Soil and Environmental Sciences, University of Agriculture, Faisalabad, Pakistan

2Land Resources Research Institute, National Agricultural Research Centre, Islamabad, Pakistan

3Department of Soil Sciences, Bahauddin Zakariya University, Multan, Pakistan

Abstract

Phosphorus (P) is 2nd most important macronutrient for the growth of plants. The soils of Pakistan are alkaline and calcareous in nature which causes precipitation of P and making it less available to the plants. To overcome this issue, excessive P fertilization is used, some part of fertilizer is used by plants while remaining is either fixed as unavailable form or lost due to runoff. So deficiency of P affects the plant growth while runoff of excess fertilizer may result in eutrophication of our aquatic systems. Use of arbuscular mycorrhizal fungi (AMF) may be an appropriate method to enhance the P uptake efficiency of plants and reduce the use of P fertilizers. Plant growth promoting rhizobacteria (PGPR) are also well known for solubilization of P. Therefore, a pot experiment was conducted in 2019 at Research Farm of Institute of Soil and Environmental Sciences, University of Agriculture Faisalabad, Pakistan to check the efficiency of compost, PGPR and AMF to enhance availability of P in onion. The impact of PGPR and AMF were evaluated separately and in combination. At harvest, data regarding plant fresh and dry weight, onion bulb circumference, bulb fresh and dry weight, chlorophyll a and b, carotenoids, and P concentration in plants were analyzed. Results showed that combined inoculation of endomycorrhizae and rhizobacteria improved plant fresh and dry weight, onion bulb circumference, bulb fresh and dry weight, chlorophyll a and b, carotenoids. Overall, integrated application of endomycorrhizae and rhizobacteria enhanced P uptake by 77% as compared to control treatment.

Keywords: PGPR, Endomycorrhizae, Compost, Phosphorus uptake, Onion

Rill treatments to enhance nutrient rich soil, a case study

Subash Thanappan*, Shashidhara Ranganath Hosamani, Mahesh Naganahalli Chandrappa

Department of Civil Engineering, Ambo University, Ambo, Ethiopia

Abstract

This article mainly focuses on the soil conservation practices to counteract the soil degradation and to maintain the soil nutrients and organic matter. As the study area is highly prone to the soil erosion, the comparison study was initiated for the rill treatment with the help of sweet gum balls, riprap (different sizes of stones), mulch with coconut shells, bermuda grass with polyacrylamic (PAM) and a control. Five different rills of same size including an untreated control with four different materials used for making the protective covers were experimented initially using the triangular notches of 600 by maintaining the actual discharge of 0.001379 m3 sec-1. The rills were continuously kept under the visual observations for about two years which includes two monsoon seasons for each year. The changes in the size of the rills after every 4 rainy seasons are documented. The results of the experiments showed that the erosion control capacity of various protective covers are 68.4, 57, 51.2 and 44.8 respectively with the ingredients riprap, sweet gum ball, bermuda grass with PAM  and the mulch with the coconut shells.

Keywords: Analytical hierarchy process, Mulch, Polyacrylamic, Rip rap, Soil erosion, Visual observations

Subash Thanappan*, Shashidhara Ranganath Hosamani, Mahesh Naganahalli Chandrappa

Department of Civil Engineering, Ambo University, Ambo, Ethiopia

A comparative study to develop calcium, zinc, and antioxidant rich drinking yoghurt products using plant and pharmaceutical ingredients

Jayani Samarathunga1*, Madhura Jayasinghe1, Maharsha Edirisinghe2, Isuru Wijesekara1, Piumi Abeysundara1, Afshan Shafi3, Umar Farooq3, Subhashinie Senadheera4

1Department of Food Science and Technology, Faculty of Applied Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka

2MILCO (Pvt) Ltd., No.45, Nawala Road, Narahenpita, Colombo, Sri Lanka

3Department of Food Science and Technology, MNS-University of Agriculture, Multan, Pakistan

4Department of Biochemistry, Faculty of Medicine and Allied Sciences, Rajarata University of Sri Lanka, Saliyapura, Sri Lanka

Abstract

Today, people in the world hungers natural foods. This study is an attempt taken to investigate the compatibility of utilizing plant sources in fortification rather than pharmaceutical sources. Two sets of drinking yoghurt products were prepared using cow’s milk and fortified using selected plant and pharmaceutical sources of Ca and Zn separately; as they fulfil 1/3, 1/2 and 2/3 of Reference Dietary Intakes (RDI) of Ca and Zn. Drinking yoghurt fortified at 50% RDI of Ca with plant extracts elicited best sensory properties among all products. Incorporation of Moringa oleifera dried leaf powder extract and Phyllanthus emblica fruit extract had significantly increased the mineral composition compared to plain drinking yoghurt (p <0.05). All drinking yoghurts incorporated with plant ingredients had significantly higher (p<0.05) antioxidant activity (Range: 52.08+0.02- 91.69+0.00%) and total phenolic contents (2.29-3.60 mg GAE/g) than the control. All products fortified with Ca and Zn using pharmaceutical sources showed significantly lower (p<0.05) antioxidant activity. Microbiological evaluation did not elicit any significant difference (p>0.05) after subjected to mineral fortification from either plant or pharmaceutical sources. During the shelf life evaluation, quick increment in pH was observed in products with pharmaceutical ingredients than the control. Syneresis was higher in samples with plant ingredients than the control and pharmaceutical ingredient added products but was not greatly affecting the product stability during two weeks of shelf life period. Overall, the incorporation of M. oleifera and P. emblica can be concluded as reliable option for dairy products such as drinking yoghurts; to fortify them with high Ca and Zn, to enhance antioxidant properties as well as to maintain product stability and organoleptic properties.

Keywords: Phyllanthus emblica, Moringa oleifera, Ca fortification, Zn fortification, Antioxidant activity