2024(2)      April- June, 2024

Antioxidant, anti-inflammatory, anti-arthritic activities and acute toxicity of Calendula stellata n-butanol extract from Algeria

Amina Foughalia1, Sakina Zerizer1*, Boutheyna Aribi1, Zahia Kabouche2, Chawki Bensouici3

1Département de Biologie Animale, Laboratoire d’Immunologie et Activités Biologiques des Substances Naturelles, Université des frères Mentouri-Constantine 1, 25000 Constantine, Algeria

2Département de Chimie, Laboratoire d’Obtention de Substances Thérapeutiques, Université des frères Mentouri-Constantine1, 25000 Constantine, Algeria

3Centre de Recherche en Biotechnologie (C.R.Bt), Ali Mendjli Nouvelle Ville BPE.73 Constantine, Alegria


Calendula stellata (Asteraceae family), growing in North-East Algeria was investigated for its biological activities in laboratory animal model studies. The n-butanol extract was prepared from aerial parts using ethanol maceration followed by liquid-liquid extraction then, the total phenolic and flavonoid contents were measured, and the antioxidant activity was evaluated using DPPH, ABTS, CUPRAC, and reducing power assay. Then acute toxicity was tested in mice using the Up and Down test and, the anti-inflammatory anti-arthritic activity was evaluated using formalin induced arthritis (FIA) in Wistar rats. Results indicated that, the extract was rich in phenolic and flavonoid contents (224.097 ±7.31 mg GAE/g and 207.36±10.081mg QE/g, respectively). It possessed considerable antioxidant activity, the extract showed no visible toxicity or mortality signs, and the LD50 was > 2000mg/kg body weight. Furthermore, in the FIA, the extract showed significant dose-dependent inhibition in paw edema. It also it decreased the C-reactive protein (CRP) plasmatic, preventing cartilage destruction and liver injury. In conclusion, C. stellata n-butanol extract possesses antioxidant and anti-arthritic activities, in addition to protective properties in hepatic tissue.


Keywords: Calendula stellata, Antioxidant, Anti-inflammatory, Anti-arthritic, Algeria

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